ABclonal

VEGF Receptor 1 Rabbit pAb

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Product name VEGF Receptor 1 Rabbit pAb
Catalog No. AB-A0058
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1100-1200 of human VEGF Receptor 1 (NP_002010.2).

Gene ID

2321

Swiss prot

P17948

Synonyms

FLT; FLT-1; VEGFR-1; VEGFR1; FLT1

Calculated MW

39kDa/41kDa/52- 82kDa/150kDa

Observed MW

200KDa

Reactivity Human, Mouse, Rat
Tested applications WB IF
Recommended dilution WB 1:200 - 1:2000
IF 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples Mouse brain, Rat brain
Cellular location Cell membrane, Cytoplasm, Endosome, Secreted, Single-pass type I membrane protein
ABclonal:Western blot - VEGF Receptor 1 Rabbit pAb (A0058) }

Western blot - VEGF Receptor 1 Rabbit pAb (A0058)

Western blot analysis of extracts of various cell lines, using VEGF Receptor 1 antibody (A0058) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - VEGF Receptor 1 Rabbit pAb (A0058) }

Immunofluorescence - VEGF Receptor 1 Rabbit pAb (A0058)

Immunofluorescence analysis of NIH/3T3 cells using VEGF Receptor 1 antibody (A0058) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - VEGF Receptor 1 Rabbit pAb (A0058) }

Immunofluorescence - VEGF Receptor 1 Rabbit pAb (A0058)

Immunofluorescence analysis of U2OS cells using VEGF Receptor 1 antibody (A0058) at dilution of 1:100. Blue: DAPI for nuclear staining.  
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