ABclonal

Bcl-2 Rabbit pAb

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  • €59,00
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Product name Bcl-2 Rabbit pAb
Catalog No. AB-A0208
Host species Rabbit
Purification method Affinity purification
Isotype IgG
Immunogen A synthetic peptide of human Bcl-2
Sequence
Gene ID 596
Swiss prot P10415
Synonyms Bcl-2; PPP1R50; BCL2
Calculated MW 22kDa/26kDa
Observed MW 26KDa
Reactivity Human, Mouse, Rat
Tested applications WB IHC IF IP
Recommended dilution WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IP 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples 293T, THP-1, HeLa
Cellular location Endoplasmic reticulum membrane, Mitochondrion outer membrane, Nucleus membrane, Single-pass membrane protein
Customer validation

(rat cells, Human skin T cell lymphoma, CAL27, Hut102, HCT116, B6, MCF-7,MD-MBA-231)

WB(Rattus norvegicus, Homo sapiens, Mus musculus, Gallus gallus, Bos taurus, Capra hircus, Ctenopharyngodon, Ctenopharyngodon idellus, Giardia duodenalis)

IHC(Homo sapiens, Rattus norvegicus, Mus musculus)

IHC(Mus musculus)

IF(Homo sapiens)

100 in total (link)

PMID: 26550949  Title: Glycyrrhizin attenuates isoflurane-induced cognitive deficits in neonatal rats via its anti-inflammatory activity  PMID: 26898450  Title: Carvedilol protects bone marrow stem cells against hydrogen peroxide-induced cell death via PI3K-AKT pathway  PMID: 26918829  Title: miR-19b attenuates H2O2-induced apoptosis in rat H9C2 cardiomyocytes via targeting PTEN  PMID: 28979702  Title: Chikusetsu saponin IVa attenuates isoflurane-induced neurotoxicity and cognitive deficits via SIRT1/ERK1/2 in developmental rats  PMID: 27924630  Title: Gelsolin-like actin-capping protein is associated with patient prognosis, cellular apoptosis and proliferation in prostate cancer  PMID: 29033244  Title: iASPP Is an Antioxidative Factor and Drives Cancer Growth and Drug Resistance by Competing with Nrf2 for Keap1 Binding  PMID: 28089662  Title: Electroacupuncture pretreatment attenuates spinal cord ischemia-reperfusion injury via inhibition of high-mobility group box 1 production in a LXA4 receptor-dependent manner  PMID: 29048657  Title: Toosendanin suppresses oncogenic phenotypes of human gastric carcinoma SGC?7901 cells partly via miR?200a?mediated downregulation of β-catenin pathway  PMID: 28878358  Title: Enoyl-CoA hydratase-1 regulates mTOR signaling and apoptosis by sensing nutrients  PMID: 29190957  Title: MicroRNA-30c-5p ameliorates hypoxia-reoxygenation-induced tubular epithelial cell injury via HIF1α stabilization by targeting SOCS3  PMID: 29050313  Title: Adipose-derived mesenchymal stem cells employed exosomes to attenuate AKI-CKD transition through tubular epithelial cell dependent Sox9 activation  PMID: 29412164  Title: SGK1 inhibits PM2. 5-induced apoptosis and oxidative stress in human lung alveolar epithelial A549 cells  PMID: 29572744  Title: Ameliorative effects of nano-elemental selenium against hexavalent chromium-induced apoptosis in broiler liver  PMID: 29736179  Title: Natural Compound Methyl Protodioscin Suppresses Proliferation and Inhibits Glycolysis in Pancreatic Cancer  PMID: 29743526  Title:  Pretreatment of Huaiqihuang extractum protects against cisplatin-induced nephrotoxicity  ......
ABclonal:Western blot - Bcl-2 Rabbit pAb (A0208) }

Western blot - Bcl-2 Rabbit pAb (A0208)

Western blot analysis of extracts of various cell lines, using Bcl-2 antibody (A0208) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Bcl-2 Rabbit pAb (A0208) }

Immunohistochemistry - Bcl-2 Rabbit pAb (A0208)

Immunohistochemistry of paraffin-embedded human appendix using Bcl-2 Rabbit pAb (A0208) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - Bcl-2 Rabbit pAb (A0208) }

Immunofluorescence - Bcl-2 Rabbit pAb (A0208)

Immunofluorescence analysis of BALB-3T3 cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Bcl-2 Rabbit pAb (A0208) }

Immunofluorescence - Bcl-2 Rabbit pAb (A0208)

Immunofluorescence analysis of C6 cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Bcl-2 Rabbit pAb (A0208) }

Immunofluorescence - Bcl-2 Rabbit pAb (A0208)

Immunofluorescence analysis of U-2 OS cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - Bcl-2 Rabbit pAb (A0208) }

Immunoprecipitation - Bcl-2 Rabbit pAb (A0208)

Immunoprecipitation analysis of 200ug extracts of THP-1 cells using 3ug Bcl-2 antibody (A0208). Western blot was performed from the immunoprecipitate using Bcl-2 antibody (A0208) at a dilition of 1:1000.
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