Ubiquitin Rabbit pAb

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Product name Ubiquitin Rabbit pAb
Catalog No. AB-A0162
Host species Rabbit
Purification method Affinity purification
Isotype IgG


A synthetic peptide of human Ubiquitin

Gene ID


Swiss prot



HEL-S-50; Ubiquitin; UBB

Calculated MW


Observed MW


Reactivity Human, Mouse
Tested applications WB
Recommended dilution WB 1:1000 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HeLa, Mouse kidney
Cellular location Cytoplasm, Nucleus
Customer validation

WB(Homo sapiens, Crassostrea gigas, Mus musculus)


PMID: 24260525  Title: Ubiquitin-specific proteases 25 negatively regulates virus-induced type I interferon signaling  PMID: 25903338  Title: Human Cytomegalovirus Infection Dysregulates the Localization and Stability of NICD1 and Jag1 in Neural Progenitor Cells  PMID: 26061460  Title: Ubiquitin-specific Protease 15 Negatively Regulates Virus-induced Type I Interferon Signaling via Catalytically-dependent and -independent Mechanisms  PMID: 29437978  Title: WDR5 Facilitates Human Cytomegalovirus Replication by Promoting Capsid Nuclear Egress  PMID: 26928091  Title: A novel ubiquitin-protein ligase E3 functions as a modulator of immune response against lipopolysaccharide in Pacific oyster, Crassostrea gigas  PMID: 31629811  Title: Characterization of PHB in the gonadal development of the swimming crab Portunus trituberculatus  PMID: 28750047  Title: Human cytomegalovirus IE1 downregulates Hes1 in neural progenitor cells as a potential E3 ubiquitin ligase.  PMID: 30389518  Title: A vital ubiquitin-conjugating enzyme CgUbe2g1 participated in regulation of immune response of Pacific oyster Crassostrea gigas.  PMID: 31865016  Title: Development of cell-permeable peptide-based PROTACs targeting estrogen receptor α 


ABclonal:Western blot - Ubiquitin Rabbit pAb (A0162) }

Western blot - Ubiquitin Rabbit pAb (A0162)

Western blot analysis of extracts of various cell lines, using Ubiquitin pAb (A0162) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.