ABclonal

TNF-α Rabbit pAb

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Product name

TNF-α Rabbit pAb

Catalog No. AB- A0277
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-233 of human TNF (NP_000585.2).

Gene ID

7124

Swiss prot

P01375

Synonyms

TNF; DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F; TNF alpha

Calculated MW

25kDa

Observed MW

17KDa

Reactivity Human, Mouse, Rat
Tested applications WB IHC IF
Recommended dilution WB 1:1000 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples THP-1, RAW264.7
Cellular location Cell membrane, Membrane, Secreted, Single-pass type II membrane protein, Single-pass type II membrane protein
Customer validation

(Mouse embryonal fibroblast cell)

WB(Rattus norvegicus, Homo sapiens, Mus musculus, Canis lupus familiaris)

ELISA(Mus musculus)

WB(Mus musculus)


PMID: 25645818  Title: Puerarin decreases bone loss and collagen destruction in rats with ligature-induced periodontitis  PMID: 26887382  Title: Prevention of Hippocampal Neuronal Damage and Cognitive Function Deficits in Vascular Dementia by Dextromethorphan  PMID: 29391237  Title: Sulforaphane attenuates microglia-mediated neuronal necroptosis through down-regulation of MAPK/NF-κB signaling pathways in LPS-activated BV-2 microglia  PMID: 29605298  Title: Micro124-mediated AHR expression regulates the inflammatory response of chronic rhinosinusitis (CRS) with nasal polyps  PMID: 30207732  Title: Synergetic and antagonistic molecular effects mediated by the feedback loop of p53 and JNK between Saikosaponin D and SP600125 on lung cancer A549 cells  PMID: 32072182  Title: Resveratrol suppresses bone cancer pain in rats by attenuating inflammatory responses through the AMPK/Drp1 signaling  PMID: 32199223  Title: DHA attenuates Aβ-induced necroptosis through the RIPK1/RIPK3 signaling pathway in THP-1 monocytes  PMID: 32319625  Title: Effects of combined treatment with PD‑L1 Ig and CD40L mAb on immune tolerance in the CBA/J x DBA/2 mouse model  PMID: 32320383  Title: Protective effects of autophagy and NFE2L2 on reactive oxygen species-induced pyroptosis of human nucleus pulposus cells  PMID: 30811965  Title: Carotid baroreceptor stimulation improves cardiac performance and reverses ventricular remodelling in canines with pacing-induced heart failure.  PMID: 31418947  Title: Inhibition of CREB-mediated ZO-1 and Activation of NF-κB-induced IL-6 by Colonic Epithelial MCT4 Destroys Intestinal Barrier Function.  PMID: 31763156  Title: Heat Shock Protein Inspired Nanochaperones Restore Amyloid-β Homeostasis for Preventative Therapy of Alzheimer's Disease.  PMID: 32987101  Title: Treadmill exercise mitigates neuroinflammation and increases BDNF via activation of SIRT1 signaling in a mouse model of T2DM  ......

 

ABclonal:Western blot - TNF-α Rabbit pAb (A0277) }

Western blot - TNF-α Rabbit pAb (A0277)

Western blot analysis of extracts of various cell lines, using TNF-α antibody (A0277) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A0277) }

Immunohistochemistry - TNF-α Rabbit pAb (A0277)

Immunohistochemistry of paraffin-embedded Human colon carcinoma using TNF-α antibody (A0277) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A0277) }

Immunohistochemistry - TNF-α Rabbit pAb (A0277)

Immunohistochemistry of paraffin-embedded Human breast cancer using TNF-α antibody (A0277) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A0277) }

Immunohistochemistry - TNF-α Rabbit pAb (A0277)

Immunohistochemistry of paraffin-embedded Human gastric cancer using TNF-α antibody (A0277) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - TNF-α Rabbit pAb (A0277) }

Immunofluorescence - TNF-α Rabbit pAb (A0277)

Immunofluorescence analysis of Human mammary cancer cells using TNF-α antibody (A0277) at dilution of 1:100. Blue: DAPI for nuclear staining.
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