ABclonal

TLR4 Rabbit pAb

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Product name TLR4 Rabbit pAb
Catalog No. AB-A0007
Host species Rabbit
Purification method Affinity purification
Isotype IgG

 

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 470-630 of human TLR4 (NP_612564.1).

Gene ID

7099

Swiss prot

O00206

Synonyms

TLR4; ARMD10; CD284; TLR-4; TOLL

Calculated MW

73kDa/91kDa/95kDa

Observed MW

120kDa 

Reactivity Human, Mouse
Tested applications WB, IF
Recommended dilution WB 1:500 - 1:2000
IF 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples A-549
Cellular location Cell membrane, Single-pass type I membrane protein
Customer validation

WB(Mus musculus)

 

PMID: 30716318  Title: Harmine mitigates LPS-induced acute kidney injury through inhibition of the TLR4-NF-κB/NLRP3 inflammasome signalling pathway in mice.  PMID: 33500729  Title: An electroporation strategy to synthesize the membrane-coated nanoparticles for enhanced anti-inflammation therapy in bone infection 
ABclonal:Western blot - TLR4 Rabbit pAb (A0007) }

Western blot - TLR4 Rabbit pAb (A0007)

Western blot analysis of extracts of A-549 cells, using TLR4 antibody (A0007) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5min.
ABclonal:Immunofluorescence - TLR4 Rabbit pAb (A0007) }

Immunofluorescence - TLR4 Rabbit pAb (A0007)

Immunofluorescence analysis of RAW264.7 cells using TLR4 Polyclonal Antibody (A0007) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - TLR4 Rabbit pAb (A0007) }

Immunofluorescence - TLR4 Rabbit pAb (A0007)

Immunofluorescence analysis of THP-1 cells using TLR4 Polyclonal Antibody (A0007) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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