TIAGEN

Taq Plus DNA Polymerase (Mixed Mg2+)

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Cat. No.  TIA-ET105-02 (500 U, 2.5 U/µL) 

                TIA-ET105-01 (250 U, 2.5 U/µL)  Datasheet

Taq Plus DNA Polymerase is a mixture of Taq and Pfu DNA polymerase. It has 5'-3'exonuclease activity and 3'-5' exonuclease activity, with the characteristics of high amplification efficiency and low mismatch rate. Compared with Taq DNA polymerase, Taq Plus DNA polymerase has the advantages of increased amplification length (simple templates can be effectively amplified up to 20kb and complex templates can be up to 10 kb), good fidelity, etc. Compared with Pfu DNA polymerase, it has the advantages of fast amplification speed and high reaction efficiency.

Activity Definition

1 Unit (U) Taq Plus DNA Polymerase activity is defined as the amount of enzyme required to incorporate 10 nmol deoxynucleotides into acid-insoluble substances at 74°C within 30 min using activated salmon sperm DNA as template/primer.

Quality Control

The purity by SDS-PAGE detection is more than 99%; No activity of exogenous nuclease is detected; Single-copy gene in human genome could be amplified effectively; No significant activity change when stored at room temperature for one week.

Main Technical Parameters

Taq Plus DNA Polymerase has 5'-3' exonuclease activity and 3'-5' exonuclease activity. PCR products can be directly cloned into TA vector. If the cloning efficiency needs to be improved, it is recommended to purify the PCR products first and perform A-tailing before cloning into TA vector.

Applications

It is commonly used for amplification of templates with high fidelity and complex structure, such as high GC content and secondary structure. In most cases, it can replace Taq DNA polymerase.

Storage

Store at -20°C in the buffer with 20 mM Tris-HCl (pH8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% Glycerol, and Stabilizers

 

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