TIAGEN

Taq Platinum DNA Polymerase (Mixed Mg2+)

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Cat. No.  TIA-ET104-02 (500 U)

                TIA-ET104-01 (250 U)  Datasheet

Taq Platinum DNA Polymerase is a chemical-modified Hot Start Taq polymerase with 3'-5' exonuclease activity and 5'-3' exonuclease activity. The enzyme activity of Taq Platinum DNA Polymerase is blocked at room temperature. Its activity can only be activated after heating at 94°C for 5-10 min, thus avoiding non-specific amplification caused by primer non-specific annealing or primer dimer at low temperature before the initial cycle of PCR reaction, and greatly improving the sensitivity and specificity of PCR reaction. In addtion, Taq Platinum DNA Polymerase has very high fidelity, which is second best to Pfu polymerase. The extension speed of DNA polymerization is faster than Pfu polymerase and the amplification efficiency is higher.

Activity Definition

1 unit (U) Taq Platinum DNA Polymerase activity is defined as the amount of enzyme required to incorporate 10 nmol deoxynucleotides into acid-insoluble substances at 74°C within 30 min using activated salmon sperm DNA as template/primer.

Quality Control

The purity by SDS-PAGE detection is more than 99%; No activity of exogenous nuclease is detected; Single-copy gene in human genome could be amplified effectively; No significant activity change when stored at room temperature for one week.

Main Technical Parameters

It has 5'-3' exonuclease activity and 3'-5' exonuclease activity, and its fidelity is next to Pfu polymerase. The extension speed of Taq Platinum Polymerase is faster than Pfu polymerase and the amplification efficiency is higher. PCR products can be directly ligated to the blunt end or cloned with TA vector. If the cloning efficiency needs to be improved, it is recommended to purify first and add 3’-dA overhangs before cloning into TA vector.

Applications

It can replace Pfu polymerase to amplify high fidelity products from complex templates such as genomes, and it is suitable for applications such as cloning of expression genes, site-specific mutations and analysis of single nucleotide polymorphism (SNP), etc.

Storage

Store at -20°C in the buffer with 20 mM Tris-HCl (pH8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% Glycerol, and Stabilizers

 

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