TIAGEN

Taq DNA Polymerase (Seperated Mg2+)

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Cat. No.    TIA-ET101-02-02 (500 U, 2.5 U/µL)

                  TIA-ET101-02-04 (500 U, 5 U/µL) 

Taq DNA Polymerase is a recombinant protein with a molecular weight of 94 kDa, which has been induced and expressed from Escherichia coli cloned with Thermo aquatica DNA Polymerase gene, and then purified and separated by three steps of column purification. The enzyme has good stability and strong specificity, with no exogenous nuclease and bacterial DNA pollution, and is suitable for routine PCR amplification.

Activity Definition

1 unit (U) Taq Platinum DNA Polymerase activity is defined as the amount of enzyme required to incorporate 10 nmol deoxynucleotides into acid-insoluble substances at 74°C within 30 min using activated salmon sperm DNA as template/primer.

Quality Control

The purity by SDS-PAGE detection is more than 99%; No activity of exogenous nuclease is detected; Single-copy gene in human genome could be amplified effectively; No significant activity change when stored at room temperature for one week.

Main Technical Parameters

The enzyme has 5'-3' polymerase activity and 5'-3' exonuclease activity, and without 3'-5' exonuclease activity. The extension speed of DNA polymerization is 1-2 kb/min at 70-75℃ . The PCR product has 3'-dA overhangs, which can be directly cloned in TA-cloning vector.

Applications

It is generally used for amplification, primer extension, sequencing, and A-tailing at the blunt end of DNA that’s <6 kb and has low fidelity requirements.

Storage

Store at -20°C in the buffer with 20 mM Tris-HCl (pH8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% Glycerol, and Stabilizers

 

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