Taq DNA Polymerase (Mg2+ Plus Buffer)

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Cat. NO. AB-RK20600 (datasheet)

Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a 5´ flap endonuclease activity. Taq DNA Polymerase was isolated from a recombinant source and offers robust and reliable reactions. It tolerates a wide range of templates and incorporates dUTP, dITP, and fluorescently-labeled nucleotides.

Featured Specification
Product Source An E.coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1.
Applications Multiplex PCR, Specialty PCR, PCR , Routine PCR
Unit Definition One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 75°C.
Storage Conditions 10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Tween 20, 0.5% NP-40, 50% Glycerol, pH 7.4 @ 25°C
Storage Temperature -20°C
Reaction Conditions 1X PCR Reaction Buffer, Mg2+ plus
Reaction Buffer or Mix 1X PCR Reaction Buffer, Mg2+ plus:
20 mM Tris-HCl, 10 mM (NH4)2SO4, 10 mM KCl, 2 mM MgSO4, 0.1% Triton X-100, pH 8.8 @ 25°C
Heat Inactivation No
5' - 3' Exonuclease Yes
3' - 5' Exonuclease No
Strand Displacement +
Resulting Ends Single-base 3´ Overhangs
Protein M.W. Theoretical 94000 daltons
Error Rate ~ 285x10-6 bases