ABclonal

Taq 2X PCR Master Mix

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Manufacturer  ABclonal

Cat. No. AB-RK20602 Datasheet

Taq DNA Polymerase possesses a 5'→3' polymerase activity and 3' adenine (A) addition activity.
Taq 2X Master Mix is an optimized ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2, KCI and stabilizers. It is ideally suited to routine PCR applications on various templates including pure DNA solutions, bacterial colonies, and cDNA products. It can amplify up to 4 kb from complex genomic DNA or up to 5 kb from lambda DNA. Applicable to PCR, colony PCR and primer extension.

Components

Taq 2X PCR Master Mix

Detailed information

Featured Specification
Applications Multiplex PCR, Specialty PCR, PCR , Routine PCR
Storage Conditions 10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Tween 20, 0.5% NP-40, 50% Glycerol, pH 7.4 @ 25°C
Storage Temperature -20°C
Reaction Buffer or Mix 1X Master Mix Composition:
10 mM Tris-HCl, 1.5 mM MgCl2, 50 mM KCl, 0.08% IPGAL630, 0.05%Tween 20, pH8.6@25°C; 200 μM dNTPs, 5% Glycerol, 25 U/ml Taq DNA Polymerase.
Heat Inactivation No
5' - 3' Exonuclease Yes
3' - 5' Exonuclease No
Strand Displacement +
Resulting Ends Single-base 3' Overhangs
Error Rate ~ 285x10-6 bases

 

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