Cat. No. AB-RK20602 Datasheet
Taq DNA Polymerase possesses a 5'→3' polymerase activity and 3' adenine (A) addition activity.
Taq 2X Master Mix is an optimized ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2, KCI and stabilizers. It is ideally suited to routine PCR applications on various templates including pure DNA solutions, bacterial colonies, and cDNA products. It can amplify up to 4 kb from complex genomic DNA or up to 5 kb from lambda DNA. Applicable to PCR, colony PCR and primer extension.
Taq 2X PCR Master Mix
|Applications||Multiplex PCR, Specialty PCR, PCR , Routine PCR|
|Storage Conditions||10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Tween 20, 0.5% NP-40, 50% Glycerol, pH 7.4 @ 25°C|
|Reaction Buffer or Mix||1X Master Mix Composition:
10 mM Tris-HCl, 1.5 mM MgCl2, 50 mM KCl, 0.08% IPGAL630, 0.05%Tween 20, pH8.6@25°C; 200 μM dNTPs, 5% Glycerol, 25 U/ml Taq DNA Polymerase.
|5' - 3' Exonuclease||Yes|
|3' - 5' Exonuclease||No|
|Resulting Ends||Single-base 3' Overhangs|
|Error Rate||~ 285x10-6 bases|