ABclonal

STAT1 Rabbit pAb

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Product name STAT1 Rabbit pAb
Catalog No. AB-A0027
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 513-712 of human STAT1 (NP_644671.1).

Gene ID

6772

Swiss prot

P42224

Synonyms

CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91; STAT1 alpha; STAT1

Calculated MW

83kDa/87kDa

Observed MW

84KDa

Reactivity Human, Mouse, Rat
Tested applications WBIHC
Recommended dilution WB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples HT-29, Mouse kidney, Mouse heart
Cellular location Cytoplasm, Nucleus
Customer validation

(293T)

WB(Homo sapiens, Mus musculus, Gallus gallus, Macaca mulatta, Cynoglossus semilaevis)

PMID: 27621415  Title: PKA regulatory IIα subunit is essential for PGD2-mediated resolution of inflammation  PMID: 28057839  Title: Niacin Promotes Cardiac Healing after Myocardial Infarction through Activation of the Myeloid Prostaglandin D2 Receptor Subtype 1  PMID: 31011625  Title: Fludarabine as an Adjuvant Improves Newcastle Disease Virus-Mediated Antitumor Immunity in Hepatocellular Carcinoma.  PMID: 31374219   Title: An endogenous retroviral element exerts an antiviral innate immune function via the derived lncRNA lnc-ALVE1-AS1.  PMID: 31076724  Title: ZBP1 mediates interferon-induced necroptosis.  PMID: 26361011  Title: iTRAQ-based comparative proteomic analysis of Vero cells infected with virulent and CV777 vaccine strain-like strains of porcine epidemic diarrhea virus  PMID: 32360948  Title: Artemisinin inhibits the replication of flaviviruses by promoting the type I interferon production  PMID: 30827402  Title: In vivo cellular and molecular study on duck spleen infected by duck Tembusu virus.  PMID: 32731335  Title: Structural and Biological Basis of Alphacoronavirus nsp1 Associated with Host Proliferation and Immune Evasion 
ABclonal:Western blot - STAT1 Rabbit pAb (A0027) }

Western blot - STAT1 Rabbit pAb (A0027)

Western blot analysis of extracts of various cell lines, using STAT1 antibody (A0027) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - STAT1 Rabbit pAb (A0027) }

 

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