ABclonal

SOD1 Rabbit pAb

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Product name

SOD1 Rabbit pAb

Catalog No. AB-A0274
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-154 of human SOD1 (NP_000445.1).

Gene ID

6647

Swiss prot

P00441

Synonyms

SOD1; ALS; ALS1; HEL-S-44; IPOA; SOD; hSod1; homodimer

Calculated MW

15kDa

Observed MW

20kDa

Reactivity Human, Mouse, Rat
Tested applications WB IHC IF
Recommended dilution WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HeLa, MCF7
Cellular location Cytoplasm, Nucleus
Customer validation

(293T)

WB(Homo sapiens, Mus musculus, Bos taurus, Rattus norvegicus)

Co-IP(Mus musculus)


PMID: 26761025  Title: The Influenza Virus H5N1 Infection Can Induce ROS Production for Viral Replication and Host Cell Death in A549 Cells Modulated by Human Cu/Zn Superoxide Dismutase (SOD1) Overexpression  PMID: 29605032  Title: GYY4137 protects against myocardial ischemia/reperfusion injury via activation of the PHLPP-1/Akt/Nrf2 signaling pathway in diabetic mice  PMID: 30986544  Title: LongShengZhi Capsule reduces carrageenan-induced thrombosis by reducing activation of platelets and endothelial cells.  PMID: 31066116  Title: MARVELD1 interacting with catalase regulates reactive oxygen species metabolism and mediates the sensitivity to chemotherapeutic drugs in epithelial tumors of the reproductive system.  PMID: 31011285  Title: The Differential Expression of Mitochondrial Function-Associated Proteins and Antioxidant Enzymes during Bovine Herpesvirus 1 Infection: A Potential Mechanism for Virus Infection-Induced Oxidative Mitochondrial Dysfunction  PMID: 26761025  Title: The Influenza Virus H5N1 Infection Can Induce ROS Production for Viral Replication and Host Cell Death in A549 Cells Modulated by Human Cu/Zn Superoxide Dismutase (SOD1) Overexpression  PMID: 29605032  Title: GYY4137 protects against myocardial ischemia/reperfusion injury via activation of the PHLPP-1/Akt/Nrf2 signaling pathway in diabetic mice  PMID: 30986544  Title: LongShengZhi Capsule reduces carrageenan-induced thrombosis by reducing activation of platelets and endothelial cells.  PMID: 31066116  Title: MARVELD1 interacting with catalase regulates reactive oxygen species metabolism and mediates the sensitivity to chemotherapeutic drugs in epithelial tumors of the reproductive system.  PMID: 31011285  Title: The Differential Expression of Mitochondrial Function-Associated Proteins and Antioxidant Enzymes during Bovine Herpesvirus 1 Infection: A Potential Mechanism for Virus Infection-Induced Oxidative Mitochondrial Dysfunction    ......
ABclonal:Western blot - SOD1 Rabbit pAb (A0274) }

Western blot - SOD1 Rabbit pAb (A0274)

Western blot analysis of extracts of various cell lines, using SOD1 antibody (A0274).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
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