ABclonal

SARS-COV-2 Nucleocapsid Protein Antibody (DET)

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Manufacturer  ABclonal

Product name SARS-COV-2 Nucleocapsid Protein Antibody (DET)
Catalog No. AB-RM17575
Catagory Primary antibody
Application ELISA
Description Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Immunogen E.coli derived SARS-COV-2 Nucleoproteins Ser2-Ala419(Gly335Ala)
Accession #QHD43423.2
Source Rabbit
Purirty Affinity purification
Ig Type Rabbit IgG
Endotoxin Level < 1.0 EU/μg of the protein by L AL method.
Formulation Supplied as a 0.2um filtered solution in PBS, pH 7.4.
Storage This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.

Avoid repeated freeze-thaw cycles.


SARS-COV-2 Nucleocapsid Sandwich Immunoassay

  Recommended
Concentration
Sample
ELISA Capture 1-4μg/mL SARS-CoV-2 Nucleocapsid Protein Antibody(CAP)(Cat. No. RM17574)
ELISA Detection 0.05-0.2μg/mL SARS-CoV-2 Nucleocapsid Protein Antibody(DET)(Cat. No. RM17575)
Standard 1.56-100pg/mL Recombinant SARS-COV-2 Nucleocapsid Protein with His tag(Cat. No. RP01264)


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This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
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No cross-reactivity in ELISA with MERS-CoV Nucleoprotein protein and SARS Coronavirus Nucleocapsid.
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Cat. No. RM17574, captured on COOH chip can bind AB1 with an affinity constant of 1.20nM as determined in a LSPR assay .





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