RNase H

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Cat. NO. AB-RK20535 (datasheet)

RNase H (Ribonuclease H) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA. This enzyme does not digest single or double-stranded DNA.

Featured Specification
Product Source An E.coli strain that carries the cloned RNase H gene (rnh) from E. coli.
Applications RT-PCR & cDNA Synthesis, RT-qPCR, RT-PCR and cDNA Synthesis, PCR
Unit Definition One unit is defined as the amount of enzyme required to produce 1 nmol of ribonucleotides from 20 picomoles of a fluorescently labelled 50 base pair RNA-DNA hybrid in a total reaction volume of 50 ul in 20 minutes at 37°C.
Storage Conditions 10 mM Tris-HCl, 50 mM KCl,0.1 mM EDTA, 1 mM DTT,200 μg/ml BSA,50% Glycerol,pH7.4 @ 25°C
Storage Temperature -20°C
Reaction Conditions 1X RNase H Reaction Buffer, Incubate at 37°C
Reaction Buffer or Mix 1X RNase H Reaction Buffer:
50 mM Tris-HCl, 75 mM KCl, 3 mM MgCl2, 10 mM dithiothreitol, pH 8.3 @ 25°C
Heat Inactivation 65°C for 20 min