ABclonal

Raf1 Rabbit pAb

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Product name Raf1 Rabbit pAb
Catalog No. AB-A0223
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 190-350 of human Raf1 (NP_002871.1).

Gene ID

5894

Swiss prot

P04049

Synonyms

CMD1NN; CRAF; NS5; Raf-1; c-Raf; RAF1

Calculated MW

73kDa/75kDa

Observed MW

73KDa

Reactivity Human, Mouse, Rat
Tested applications WB IHC IF IP
Recommended dilution WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples Mouse liver
Cellular location Cell membrane, Cytoplasm, Mitochondrion, Nucleus
Customer validation

(Human lung, Murine cells, Mouse spleen)

WB(Homo sapiens, Rattus norvegicus, Mus musculus)


PMID: 29377576  Title: Wogonoside induces depalmitoylation and translocation of PLSCR1 and N‐RAS in primary acute myeloid leukaemia cells  PMID: 31754334  Title: MiR-93 regulates vascular smooth muscle cell proliferation, and neointimal formation through targeting Mfn2  PMID: 30365123  Title: Dendritic cell factor 1 inhibits proliferation and migration and induces apoptosis of neuroblastoma cells by inhibiting the ERK signaling pathway. 
ABclonal:Western blot - Raf1 Rabbit pAb (A0223) }

Western blot - Raf1 Rabbit pAb (A0223)

Western blot analysis of extracts of Mouse liver, using Raf1 antibody (A0223) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Raf1 Rabbit pAb (A0223) }

Immunohistochemistry - Raf1 Rabbit pAb (A0223)

Immunohistochemistry of paraffin-embedded human colon carcinoma using Raf1 Rabbit pAb (A0223) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - Raf1 Rabbit pAb (A0223) }

Immunofluorescence - Raf1 Rabbit pAb (A0223)

Immunofluorescence analysis of HeLa cells using Raf1 Rabbit pAb (A0223) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Raf1 Rabbit pAb (A0223) }

Immunofluorescence - Raf1 Rabbit pAb (A0223)

Immunofluorescence analysis of NIH-3T3 cells using Raf1 Rabbit pAb (A0223) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - Raf1 Rabbit pAb (A0223) }

Immunoprecipitation - Raf1 Rabbit pAb (A0223)

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Raf1 antibody (A0223). Western blot was performed from the immunoprecipitate using Raf1 antibody (A0223) at a dilition of 1:1000.
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