ABclonal

PPAR gamma Rabbit pAb

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Product name

PPAR gamma Rabbit pAb

Catalog No. AB-A0270
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human PPAR gamma (NP_056953.2).

Gene ID

5468

Swiss prot

P37231

Synonyms

CIMT1; GLM1; NR1C3; PPARG1; PPARG2; PPARgamma; PPAR gamma; PPARG

Calculated MW

21kDa/54kDa/57kDa

Observed MW

57kDa

Reactivity Human, Mouse, Rat
Tested applications WB IHC IF
Recommended dilution WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples SKOV3, THP-1, SW620, Mouse kidney
Cellular location Cytoplasm, Nucleus
Customer validation

(Mouse Embryonic Stem Cells, Human liver cell)

WB(Mus musculus, Rattus norvegicus, Mesocricetus auratus, Homo sapiens, Sus scrofa)

IF(Homo sapiens)

IHC(Mus musculus)


PMID: 26673543  Title: The peroxisome proliferator-activated receptor γ agonist pioglitazone prevents NF-κB activation in cisplatin nephrotoxicity through the reduction of p65 acetylation via the AMPK-SIRT1/p300 pathway  PMID: 30607811  Title: MALAT1 Up-Regulator Polydatin Protects Brain Microvascular Integrity and Ameliorates Stroke Through C/EBPβ/MALAT1/CREB/PGC-1α/PPARγ Pathway.  PMID: 31025261  Title: Phenotypic characterization of a novel type 2 diabetes animal model in a SHANXI MU colony of Chinese hamsters.  PMID: 32000827  Title: Simvastatin re-sensitizes hepatocellular carcinoma cells to sorafenib by inhibiting HIF-1α/PPAR-γ/PKM2-mediated glycolysis  PMID: 27385003  Title: Overexpression of AKR1C3 significantly enhances human prostate cancer cells resistance to radiation.  PMID: 32467715  Title: Resistant dextrin improves high-fat-high-fructose diet induced insulin resistance.  PMID: 32917723  Title: Cadmium-mediated lung injury is exacerbated by the persistence of classically activated macrophages  PMID: 33485120  Title: The new coumarin compound Bis 3 ameliorates cognitive disorder and suppresses brain-intestine-liver systematic oxidative stress in high-fat diet mice  PMID: 32171241  Title: miR-34a regulates adipogenesis in porcine intramuscular adipocytes by targeting ACSL4  PMID: 33623411  Title: Securidaca inappendiculata-Derived Xanthones Protected Joints from Degradation in Male Rats with Collagen-Induced Arthritis by Regulating PPAR-γ Signaling    ......

 

ABclonal:Western blot - PPAR gamma Rabbit pAb (A0270) }

Western blot - PPAR gamma Rabbit pAb (A0270)

Western blot analysis of extracts of various cell lines, using PPAR gamma antibody (A0270) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - PPAR gamma Rabbit pAb (A0270) }

Immunohistochemistry - PPAR gamma Rabbit pAb (A0270)

Immunohistochemistry of paraffin-embedded Rat heart using PPAR antibody (A0270) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - PPAR gamma Rabbit pAb (A0270) }

Immunohistochemistry - PPAR gamma Rabbit pAb (A0270)

Immunohistochemistry of paraffin-embedded Human colon using PPAR antibody (A0270) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - PPAR gamma Rabbit pAb (A0270) }

Immunohistochemistry - PPAR gamma Rabbit pAb (A0270)

Immunohistochemistry of paraffin-embedded Mouse lung using PPAR antibody (A0270) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - PPAR gamma Rabbit pAb (A0270) }

Immunofluorescence - PPAR gamma Rabbit pAb (A0270)

Immunofluorescence analysis of U2OS cells using PPAR gamma antibody (A0270) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PPAR gamma Rabbit pAb (A0270) }

Immunofluorescence - PPAR gamma Rabbit pAb (A0270)

Confocal immunofluorescence analysis of HeLa cells using PPAR gamma Polyclonal Antibody (A0270) at dilution of 1:200. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PPAR gamma Rabbit pAb (A0270) }

Immunofluorescence - PPAR gamma Rabbit pAb (A0270)

Confocal immunofluorescence analysis of U-2 OS cells using PPAR gamma Polyclonal Antibody (A0270) at dilution of 1:200. Blue: DAPI for nuclear staining.
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