ABclonal

phi29-fast DNA polymerase

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Cat. NO. AB-RK21003 (datasheet)

phi29 DNA Polymerase is a replicative polymerase from the Bacillus subtilis phage phi29. phi29-fast DNA Polymerase has been genetically engineered to enhance the amplification efficiency by fusing DNA binding domains to the polymerase.
phi29-fast DNA Polymerase has exceptional strand displacement and processive synthesis properties. The polymerase has an inherent 3'→5' proofreading exonuclease activity. Compared with the traditional phi29 DNA polymerase, phi29-fast DNA polymerase has displayed an improved and faithful multiply primed DNA amplification proficiency on both circular plasmids and genomic DNA.
phi29-fast DNA Polymerase has advantaged in replication requiring a high degree of strand displacement and/or processive synthesis, as well as high fidelity and efficient replication at moderate temperatures.

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Specification

Applications

Isothermal Amplification

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C.

Storage Conditions

10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Twee-20, 0.5% (v/v) NP-40, 50% Glycerol, pH 7.5 @ 25°C

Storage Temperature

-20°C

Reaction Conditions

1X phi29 DNA Polymerase Reaction Buffer, Incubate at 30°C.

Reaction Buffer or Mix

1X phi29 DNA Polymerase Reaction Buffer:
50 mM Tris-HCl, 10 mM (NH4)2SO4, 10 mM MgCl2, 4 mM DTT, pH7.5 @ 25°C.

Heat Inactivation

65°C for 10 min

5' - 3' Exonuclease

No

3' - 5' Exonuclease

Yes

 

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