phi29 DNA Polymerase

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Cat. NO. AB-RK21002 (datasheet)

phi29 DNA Polymerase is a replicative polymerase from the Bacillus subtilis phage phi29. phi29 DNA Polymerase has exceptional strand displacement and processive synthesis properties. The polymerase has an inherent 3'→5' proofreading exonuclease activity. phi29 DNA Polymerase has advantaged in replication requiring a high degree of strand displacement and/or processive synthesis, as well as high fidelity replication at moderate temperatures.

Featured Specification
Applications Isothermal Amplification
Unit Definition One unit is defined as the amount of enzyme that will incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C.
Storage Conditions 10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Twee-20, 0.5% (v/v) NP-40, 50% Glycerol, pH 7.5 @ 25°C
Storage Temperature -20°C
Reaction Conditions 1X phi29 DNA Polymerase Reaction Buffer, Incubate at 30°C.
Reaction Buffer or Mix 1X phi29 DNA Polymerase Reaction Buffer:
50 mM Tris-HCl, 10 mM (NH4)2SO4, 10 mM MgCl2, 4 mM DTT, pH7.5 @ 25°C.
Heat Inactivation 65°C for 10 min
5' - 3' Exonuclease No
3' - 5' Exonuclease Yes
Strand Displacement +
Protein M.W. Theoretical 67000 daltons