Cat. NO. AB-RK21002 (datasheet)
phi29 DNA Polymerase is a replicative polymerase from the Bacillus subtilis phage phi29. phi29 DNA Polymerase has exceptional strand displacement and processive synthesis properties. The polymerase has an inherent 3'→5' proofreading exonuclease activity. phi29 DNA Polymerase has advantaged in replication requiring a high degree of strand displacement and/or processive synthesis, as well as high fidelity replication at moderate temperatures.
|Unit Definition||One unit is defined as the amount of enzyme that will incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C.|
|Storage Conditions||10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Twee-20, 0.5% (v/v) NP-40, 50% Glycerol, pH 7.5 @ 25°C|
|Reaction Conditions||1X phi29 DNA Polymerase Reaction Buffer, Incubate at 30°C.|
|Reaction Buffer or Mix||1X phi29 DNA Polymerase Reaction Buffer:
50 mM Tris-HCl, 10 mM (NH4)2SO4, 10 mM MgCl2, 4 mM DTT, pH7.5 @ 25°C.
|Heat Inactivation||65°C for 10 min|
|5' - 3' Exonuclease||No|
|3' - 5' Exonuclease||Yes|
|Protein M.W.||Theoretical 67000 daltons|