ABclonal

PARP1 Rabbit pAb

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Product name PARP1 Rabbit pAb
Catalog No. AB-A0010
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 150-250 of human PARP1 (NP_001609.2).

Gene ID

142

Swiss prot

P09874

Synonyms

ADPRT; ADPRT 1; ADPRT1; ARTD1; PARP; PARP-1; PPOL; pADPRT-1; PARP1; Cleaved PARP; PARP1

Calculated MW

113kDa

Observed MW

116kDa

Reactivity Human, Mouse, Rat
Tested applications WBIHCIF
Recommended dilution WB 1:1000 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples Jurkat, HeLa, RAW264.7, C6, Mouse testis, Mouse brain
Cellular location Nucleus, nucleolus
Customer validation

WB(Homo sapiens,Mus musculus)

PMID: 33391463  Title:  LT-171-861, a novel FLT3 inhibitor, shows excellent preclinical efficacy for the treatment of FLT3 mutant acute myeloid leukemia 
ABclonal:Western blot - PARP1 Rabbit pAb (A0010) }

Western blot - PARP1 Rabbit pAb (A0010)

Western blot analysis of extracts of various cell lines, using PARP1 antibody (A0010) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - PARP1 Rabbit pAb (A0010) }

Western blot - PARP1 Rabbit pAb (A0010)

Western blot analysis of extracts of various cell lines, using PARP1 antibody (A0010) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - PARP1 Rabbit pAb (A0010) }

Immunohistochemistry - PARP1 Rabbit pAb (A0010)

Immunohistochemistry of paraffin-embedded rat testis using PARP1 antibody (A0010) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - PARP1 Rabbit pAb (A0010) }

Immunohistochemistry - PARP1 Rabbit pAb (A0010)

Immunohistochemistry of paraffin-embedded human tonsil using PARP1 antibody (A0010) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - PARP1 Rabbit pAb (A0010) }

Immunohistochemistry - PARP1 Rabbit pAb (A0010)

Immunohistochemistry of paraffin-embedded mouse kidney using PARP1 antibody (A0010) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - PARP1 Rabbit pAb (A0010) }
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