ABclonal

p53 Rabbit pAb

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Product name p53 Rabbit pAb
Catalog No. AB- A0263
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human p53 (NP_000537.3).

Gene ID

7157

Swiss prot

P04637

Synonyms

BCC7; LFS1; P53; TRP53; p53; TP53; BMFS5

Calculated MW

23-43kDa

Observed MW

53KDa

Reactivity Human, Rat
Tested applications WB IHC IF IP ChIP
Recommended dilution WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
ChIP 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples 293T
Cellular location Cytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body
Customer validation

(Human cells, SW480)

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Gallus gallus, Danio rerio)

IHC(Rattus norvegicus)

IHC(Mus musculus)


PMID: 24133586  Title: Systematic screen with kinases inhibitors reveals kinases play distinct roles in growth of osteoprogenitor cells  PMID: 24427328  Title: KAT5 silencing induces apoptosis of GBC-SD cells through p38MAPK-mediated upregulation of cleaved Casp9  PMID: 23940708  Title: Comparison of toxicity of benzene metabolite hydroquinone in hematopoietic stem cells derived from murine embryonic yolk sac and adult bone marrow  PMID: 30172000  Title: Excessive apoptosis and disordered autophagy flux contribute to the neurotoxicity induced by high iodine in Sprague-Dawley rat.  PMID: 30745821  Title: NFAT5 is Regulated by p53/miR-27a Signal Axis and Promotes Mouse Ovarian Granulosa Cells Proliferation.  PMID: 32347436  Title: Upregulation of Excision Repair Cross-Complementation Group 6-Like (ERCC6L) Promotes Tumor Growth in Hepatocellular Carcinoma  PMID: 32096479  Title: Adipose-derived mesenchymal stem cells-derived exosome-mediated microRNA-342-5p protects endothelial cells against atherosclerosis  PMID: 31217873  Title: Effect of astaxanthin on apoptosis of rat renal tubular epithelial cells induced by iohexol.  PMID: 32099972  Title: Finding an efficient tetramethylated hydroxydiethylene of resveratrol analogue for potential anticancer agent  PMID: 31814908  Title: A pilot study comparing the genetic molecular biology of gestational and non-gestational choriocarcinoma  PMID: 30679505  Title: Usp7 regulates Hippo pathway through deubiquitinating the transcriptional coactivator Yorkie.  PMID: 29158805  Title: Histone Methyltransferase SETDB1 Promotes the Progression of Colorectal Cancer by Inhibiting the Expression of TP53.  PMID: 30857375  Title: Proanthocyanidins Alleviates AflatoxinB1-Induced Oxidative Stress and Apoptosis through Mitochondrial Pathway in the Bursa of Fabricius of Broilers.  PMID: 31281315  Title: The Molecular Evolution and Functional Divergence of Lamprey Programmed Cell Death Genes.  PMID: 31920551  Title: Inhibition of DRP-1-Dependent Mitophagy Promotes Cochlea Hair Cell Senescence and Exacerbates Age-Related Hearing Loss.  ......

 

ABclonal:Immunohistochemistry - p53 Rabbit pAb (A0263) }

Immunohistochemistry - p53 Rabbit pAb (A0263)

Immunohistochemistry of paraffin-embedded human colon carcinoma using p53 Rabbit pAb (A0263) at dilution of 1:150 (40x lens).
ABclonal:Immunofluorescence - p53 Rabbit pAb (A0263) }

Immunofluorescence - p53 Rabbit pAb (A0263)

Confocal immunofluorescence analysis of Hela cells using p53 Polyclonal Antibody (A0263) at dilution of 1:200. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - p53 Rabbit pAb (A0263) }

Immunofluorescence - p53 Rabbit pAb (A0263)

Confocal immunofluorescence analysis of U-2 OS cells using p53 Polyclonal Antibody (A0263) at dilution of 1:200. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - p53 Rabbit pAb (A0263) }

Immunoprecipitation - p53 Rabbit pAb (A0263)

Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug p53 antibody (A0263). Western blot was performed from the immunoprecipitate using p53 antibody (A0263) at a dilition of 1:2000.
ABclonal:Chromatin Immunoprecipitation - p53 Rabbit pAb (A0263) }

Chromatin Immunoprecipitation - p53 Rabbit pAb (A0263)

Chromatin immunoprecipitation analysis of extracts of 293T cells, using p53 antibody (A0263) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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