ABclonal

NUMB Rabbit pAb

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  • €59,00
  • Regular price €101,00
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Manufacture

ABclonal

Product name

NUMB Rabbit pAb

Catalog No.

AB-A9352

Host species

Rabbit

Purification method

Affinity purification

Isotype

IgG

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 521-640 of human NUMB (NP_003735.3)

Gene ID

8650

Swiss prot

P49757

Synonyms

NUMB; C14orf41; S171; c14_5527

Calculated MW

37kDa/48-70kDa

Observed MW

71KDa

Reactivity

Human, Mouse, Rat

Tested applications

WB, IF

Recommended dilution

WB 1:500 - 1:2000
IF 1:50 - 1:200

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.

Positive samples

HeLa, LO2, U-87MG, HT-1080, SW480, A-549, Rat liver

Customer validation


ABclonal:Western blot - NUMB Polyclonal Antibody (A9352) }

Western blot - NUMB Polyclonal Antibody (A9352)

Western blot analysis of extracts of various cell lines, using NUMB antibody (A9352) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunofluorescence - NUMB Polyclonal Antibody (A9352) }

Immunofluorescence - NUMB Polyclonal Antibody (A9352)

Immunofluorescence analysis of L929 cells using NUMB antibody (A9352) at dilution of 1:100. Blue: DAPI for nuclear staining.
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