ABclonal

NEFM Rabbit mAb

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  • €69,00
  • Regular price €140,00
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Manufacture

ABclonal

Product name

NEFM Rabbit mAb

Catalog No.

AB-A19085

Host species

Rabbit

Purification method

Affinity purification

Isotype

IgG

Immunogen

A synthesized peptide derived from human NEFM

Gene ID

4741

Swiss prot

P07197

Synonyms

NEF3; NF-M; NFM; NEFM

Calculated MW

160kDa

Observed MW

160kDa

Reactivity

Human, Mouse, Rat

Tested applications

WB, IF

Recommended dilution

WB 1:500 - 1:2000
IF 1:50 - 1:200

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.

Positive samples

293T, SH-SY5Y

Customer validation

 

ABclonal:Western blot - NEFM Rabbit mAb (A19085) }

Western blot - NEFM Rabbit mAb (A19085)

Western blot analysis of extracts of various cell lines, using NEFM antibody (A19085) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - NEFM Rabbit mAb (A19085) }

Immunofluorescence - NEFM Rabbit mAb (A19085)

Immunofluorescence analysis of SH-SY5Y cells using NEFM Rabbit mAb (A19085) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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