ABclonal

MAP2K2 Rabbit pAb

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Product name MAP2K2 Rabbit pAb
Catalog No. AB- A0253
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP2K2 (NP_109587.1).

Gene ID

5605

Swiss prot

P36507

Synonyms

CFC4; MAPKK2; MEK2; MKK2; PRKMK2; MAP2K2

Calculated MW

44kDa

Observed MW

53kDa

Reactivity Human, Mouse
Tested applications WB IHC
Recommended dilution WB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples Mouse brain
Cellular location Cytoplasm, Membrane, Peripheral membrane protein
Customer validation

(Murine cells)


ABclonal:Western blot - MAP2K2 Polyclonal Antibody (A0253) }

Western blot - MAP2K2 Polyclonal Antibody (A0253)

Western blot analysis of extracts of mouse brain, using MAP2K2 antibody (A0253).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunohistochemistry - MAP2K2 Polyclonal Antibody (A0253) }

Immunohistochemistry - MAP2K2 Polyclonal Antibody (A0253)

Immunohistochemistry of paraffin-embedded human kidney using MAP2K2 antibody (A0253) at dilution of 1:100 (40x lens).
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