ABclonal

MAP2K1 Rabbit pAb

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Product name MAP2K1 Rabbit pAb
Catalog No. AB- A0252
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP2K1 (NP_002746.1).

Gene ID

5604

Swiss prot

Q02750

Synonyms

CFC3; MAPKK1; MEK1; MKK1; PRKMK1; MAP2K1

Calculated MW

40kDa/43kDa

Observed MW

46kDa

Reactivity Human, Mouse, Rat
Tested applications WB IHC IF
Recommended dilution WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples Mouse lung, Mouse stomach, Mouse brain
Cellular location Cytoplasm, Membrane, Nucleus, Peripheral membrane protein, centrosome, cytoskeleton, microtubule organizing center, spindle pole body
Customer validation

(Murine cells, U87SC)

WB(Homo sapiens, Cyprinus carpio, Rattus norvegicus)

IF(Anatinae)


PMID: 29079719  Title: Integrated proteomic and transcriptomic analysis reveals long noncoding RNA HOTAIR promotes hepatocellular carcinoma cell proliferation by regulating opioid growth factor receptor (OGFr)  PMID: 32063900  Title: Isolation and Selection of Duck Primary Cells as Pathogenic and Innate Immunologic Cell Models for Duck Plague Virus  PMID: 31879111  Title: Avermectin inhibits neutrophil extracellular traps release by activating PTEN demethylation to negatively regulate the PI3K-ERK pathway and reducing respiratory burst in carp  PMID: 30538214  Title: Involvement of Activation of Mitogen-Activated Protein Kinase (MAPK)/Extracellular Signal-Regulated Kinase (ERK) Signaling Pathway in Proliferation of Urethral Plate Fibroblasts in Finasteride-Induced Rat Hypospadias. 
ABclonal:Western blot - MAP2K1 Rabbit pAb (A0252) }

Western blot - MAP2K1 Rabbit pAb (A0252)

Western blot analysis of extracts of various cell lines, using MAP2K1 antibody (A0252).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunohistochemistry - MAP2K1 Rabbit pAb (A0252) }

Immunohistochemistry - MAP2K1 Rabbit pAb (A0252)

Immunohistochemistry of paraffin-embedded rat lung using MAP2K1 antibody (A0252) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - MAP2K1 Rabbit pAb (A0252) }

Immunofluorescence - MAP2K1 Rabbit pAb (A0252)

Immunofluorescence analysis of U2OS cells using MAP2K1 antibody (A0252). Blue: DAPI for nuclear staining.

 

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