ABclonal

LRP5 Rabbit pAb

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Product name LRP5 Rabbit pAb
Catalog No. AB-A0130
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1000-1250 of human LRP5 (NP_002326.2).

Gene ID

4041

Swiss prot

O75197

Synonyms

LRP5; BMND1; EVR1; EVR4; HBM; LR3; LRP-5; LRP7; OPPG; OPS; OPTA1; VBCH2

Calculated MW

179kDa

Observed MW

150kDa

Reactivity Human, Mouse, Rat
Tested applications WB IHC
Recommended dilution WB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples Mouse liver, Rat liver
Cellular location Endoplasmic reticulum, Membrane, Single-pass type I membrane protein
Customer validation

WB(Mus musculus, Rattus norvegicus)

PMID: 27456852  Title: Wnt5a mediated canonical Wnt signaling pathway activation in orthodontic tooth movement: possible role in the tension force-induced bone formation  PMID: 32078745  Title: Desalted duck egg white peptides promoted osteogenesis via wnt/β-catenin signal pathway  PMID: 32088641  Title: MiR-145-5p mitigates dysregulated Wnt1/β-catenin signaling pathway in rheumatoid arthritis  PMID: 32068029  Title: Berberine encapsulated PEG-coated liposomes attenuate Wnt1/β-catenin signaling in rheumatoid arthritis via miR-23a activation 

 

ABclonal:Western blot - LRP5 Rabbit pAb (A0130) }

Western blot - LRP5 Rabbit pAb (A0130)

Western blot analysis of extracts of various cell lines, using LRP5 antibody (A0130) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - LRP5 Rabbit pAb (A0130) }

Immunohistochemistry - LRP5 Rabbit pAb (A0130)

Immunohistochemistry of paraffin-embedded human liver using LRP5 antibody (A0130) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - LRP5 Rabbit pAb (A0130) }

Immunohistochemistry - LRP5 Rabbit pAb (A0130)

Immunohistochemistry of paraffin-embedded human prostate using LRP5 antibody (A0130) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - LRP5 Rabbit pAb (A0130) }

Immunohistochemistry - LRP5 Rabbit pAb (A0130)

Immunohistochemistry of paraffin-embedded mouse lung using LRP5 antibody (A0130) at dilution of 1:200 (40x lens).

 

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