TIAGEN

Long Taq DNA Polymerase (Mixed Mg2+)

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Cat. No.    TIA-ET103-02  (500 U, 2.5 U/µL)

                  TIA-ET103-01  (250 U, 2.5 U/µL)  Datasheet

Long Taq DNA Polymerase is a Taq DNA polymerase developed by TIANGEN with 5'-3' exonuclease activity and 3'-5' exonuclease activity. It has the characteristics of high amplification efficiency and high fidelity. Equipped with two efficient amplification Buffer, it can adapt to the amplification of different templates. For simple templates, it can amplify up to 40 kb of fragments, and for complex secondary structures (GC rich, etc.) and templates with repetitive sequences, it can amplify up to 15 kb of fragments.

Activity Definition

1 unit (U) Taq Platinum DNA Polymerase activity is defined as the amount of enzyme required to incorporate 10 nmol deoxynucleotides into acid-insoluble substances at 74°C within 30 min using activated salmon sperm DNA as template/primer.

Quality Control

The purity by SDS-PAGE detection is more than 99%; No activity of exogenous nuclease is detected; Single-copy gene in human genome could be amplified effectively; No significant activity change when stored at room temperature for one week.

Main Technical Parameters

It has 5'-3' exonuclease activity and 3'-5' exonuclease activity. PCR products can be directly cloned into TA vector. If the cloning efficiency needs to be improved, it is recommended to purify the PCR products first and add with 3'-dA overhang before cloning into TA vector.

Applications

It is suitable for amplification of long fragments with high fidelity requirements and some complex templates (such as secondary structure, rich GC sequence and repeat sequence, etc.), such as gene mapping, sequencing and molecular genetics research, etc.

Storage

Store at -20°C in the buffer with 20 mM Tris-HCl (pH8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% Glycerol, and Stabilizers

 

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