ABclonal

LC3A/LC3B Rabbit pAb

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Manufacture

ABclonal

Product name

LC3A/LC3B Rabbit pAb

Catalog No.

AB-A5618

Host species

Rabbit

Purification method

Affinity purification

Isotype

IgG

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-121 of human LC3A (NP_115903.1)

Gene ID

84557/81631

Swiss prot

Q9GZQ8/Q9H492

Synonyms

MAP1LC3B; ATG8F; LC3B; MAP1A/1BLC3; MAP1LC3B-a; MAP1LC3A; ATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3; LC3A/LC3B

Calculated MW

14kDa

Observed MW

14kDa/16kDa

Reactivity

Human, Mouse, Rat

Tested applications

WB, IHC, IF

Recommended dilution

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.

Positive samples

293T, SH-SY5Y, A375, Mouse brain, Rat brain, NIH/3T3, C6

Customer validation

WB(Gallus gallus, Rattus norvegicus, Homo sapiens)

PMID: 29035754  Title: Regulation of autophagy factors by oxidative stress and cardiac enzymes imbalance during arsenic or/and copper induced cardiotoxicity in Gallus gallus.  PMID: 31111379  Title: Berberine mitigates IL-21/IL-21R mediated autophagic influx in fibroblast-like synoviocytes and regulates Th17/Treg imbalance in rheumatoid arthritis.  PMID: 31452705  Title: Effect of metformin on the proliferation, apoptosis, invasion and autophagy of ovarian cancer cells.   
ABclonal:Western blot - LC3A/LC3B Rabbit pAb (A5618) }

Western blot - LC3A/LC3B Rabbit pAb (A5618)

Western blot analysis of extracts of various cell lines, using LC3A/LC3B antibody (A5618) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Western blot - LC3A/LC3B Rabbit pAb (A5618) }

Western blot - LC3A/LC3B Rabbit pAb (A5618)

Western blot analysis of extracts of various cell lines, using LC3A/LC3B antibody (A5618) at 1:500 dilution.293T cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.C6 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
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