ABclonal

[KO Validated] PARK7 Rabbit pAb

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Manufacture

ABclonal

Product name

[KO Validated] PARK7 Rabbit pAb

Catalog No.

AB-A0987

Host species

Rabbit

Purification method

Affinity purification

Isotype

IgG

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-189 of human PARK7 (NP_001116849.1)

Gene ID

11315

Swiss prot

Q99497

Synonyms

DJ-1; DJ1; GATD2; HEL-S-67p; PARK7

Calculated MW

19kDa

Observed MW

20kDa

Reactivity

Human, Mouse

Tested applications

WB, IF

Recommended dilution

WB 1:500 - 1:2000
IF 1:50 - 1:200

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.

Positive samples

HeLa, NIH/3T3, Jurkat

Customer validation

 


ABclonal:Western blot - [KO Validated] PARK7 Rabbit pAb (A0987) }

Western blot - [KO Validated] PARK7 Rabbit pAb (A0987)

Western blot analysis of extracts of various cell lines, using PARK7 antibody (A0987) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Western blot - [KO Validated] PARK7 Rabbit pAb (A0987) }

Western blot - [KO Validated] PARK7 Rabbit pAb (A0987)

Western blot analysis of extracts from normal (control) and PARK7 knockout (KO) 293T cells, using PARK7 antibody (A0987) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunofluorescence - [KO Validated] PARK7 Rabbit pAb (A0987) }

Immunofluorescence - [KO Validated] PARK7 Rabbit pAb (A0987)

Immunofluorescence analysis of HeLa cells using PARK7 antibody (A0987). Blue: DAPI for nuclear staining.
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