ABclonal

[KO Validated] NOTCH1 Rabbit pAb

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Manufacture

ABclonal

Product name

[KO Validated] NOTCH1 Rabbit pAb

Catalog No.

AB-A7636

Host species

Rabbit

Purification method

Affinity purification

Isotype

IgG

Immunogen

A synthetic peptide of human NOTCH1

Gene ID

4851

Swiss prot

P46531

Synonyms

AOS5; AOVD1; TAN1; hN1; NOTCH1; Activated NOTCH1; notch 1

Calculated MW

272kDa

Observed MW

300KDa

Reactivity

Human, Mouse, Rat

Tested applications

WB, IHC, IF, 

Recommended dilution

WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.

Positive samples

MCF-7, 293T, OVCAR3, Mouse thymus

Customer validation

(Human skin T cell lymphoma, HL60,Hut102)

WB(Homo sapiens)

PMID:32633374  Title: Linc00324 promotes the progression of papillary thyroid cancer via regulating Notch signaling pathway   

Product Images

ABclonal:Western blot - [KO Validated] NOTCH1 Polyclonal Antibody (A7636) }

Western blot - [KO Validated] NOTCH1 Polyclonal Antibody (A7636)

Western blot analysis of extracts of various cell lines, using NOTCH1 antibody (A7636) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Western blot - [KO Validated] NOTCH1 Polyclonal Antibody (A7636) }

Western blot - [KO Validated] NOTCH1 Polyclonal Antibody (A7636)

Western blot analysis of extracts from normal (control) and NOTCH1 knockout (KO) HeLa cells, using NOTCH1 antibody (A7636) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - [KO Validated] NOTCH1 Polyclonal Antibody (A7636) }

Immunohistochemistry - [KO Validated] NOTCH1 Polyclonal Antibody (A7636)

Immunohistochemistry of paraffin-embedded rat spleen using NOTCH1 antibody (A7636) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] NOTCH1 Polyclonal Antibody (A7636) }

Immunofluorescence - [KO Validated] NOTCH1 Polyclonal Antibody (A7636)

Immunofluorescence analysis of U2OS cells using NOTCH1 antibody (A7636) at dilution of 1:100. Blue: DAPI for nuclear staining.
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