ABclonal

[KO Validated] NFKB1 Rabbit pAb

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Manufacture

ABclonal

Product name

[KO Validated] NFKB1 Rabbit pAb

Catalog No.

AB-A6667

Host species

Rabbit

Purification method

Affinity purification

Isotype

IgG

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 740-964 of human NFKB1 (NP_001158884.1)

Gene ID

4790

Swiss prot

P19838

Synonyms

NFKB1; CVID12; EBP-1; KBF1; NF-kB1; NF-kappa-B; NF-kappaB; NFKB-p105; NFKB-p50; NFkappaB; p105; p50

Calculated MW

85kDa/105kDa

Observed MW

50kDa

Reactivity

Human, Mouse, Rat

Tested applications

WB, IHC, IP

Recommended dilution

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.

Positive samples

HeLa

Customer validation

(BMDC, HCC827)

WB(Mus musculus, Homo sapiens, Rattus norvegicus)

IF(Mus musculus)

IHC(Mus musculus)

PMID: 29311802  Title: Toll-like receptor 4 mediates methamphetamine-induced neuroinflammation through Caspase-11 signaling pathway in astrocytes  PMID: 28179530  Title: Japanese Encephalitis Virus NS5 Inhibits Type I Interferon (IFN) Production by Blocking the Nuclear Translocation of IFN Regulatory Factor 3 and NF-κB  PMID: 30926388  Title: TRIB1 induces macrophages to M2 phenotype by inhibiting IKB-zeta in prostate cancer.  PMID: 32007274   Title: Food with calorie restriction reduces the development of atherosclerosis in apoE-deficient mice  PMID: 32270801  Title: Molecular mechanisms of selenium-biofortified soybean protein and polyphenol conjugates in protecting mouse skin damaged by UV-B  PMID: 32273812   Title: Liraglutide attenuates gefitinib-induced cardiotoxicity and promotes cardioprotection through the regulation of MAPK/NF-κB signaling pathways  PMID: 32181695  Title: Schisandra Chinensis Acidic Polysaccharide Improves the Insulin Resistance in Type 2 Diabetic Rats by Inhibiting Inflammation  PMID: 32105766  Title: Dishevelled-1 regulated apoptosis through NF-κB in cerebral ischemia/reperfusion injury in rat PMID: 31938969  Title: Human umbilical cord mesenchymal stem cells alleviate inflammatory bowel disease by inhibiting ERK phosphorylation in neutrophils  PMID: 31049763  Title: Lactobacillus rhamnosus GG Ameliorates Liver Injury and Hypoxic Hepatitis in Rat Model of CLP-Induced Sepsis.  PMID: 30123110  Title: Role of CXCR1 and Interleukin-8 in Methamphetamine-Induced Neuronal Apoptosis.  PMID: 32295141  Title: Aerobic Exercise Inhibits CUMS-Depressed Mice Hippocampal Inflammatory Response via Activating Hippocampal miR-223/TLR4/MyD88-NF-κB Pathway. 
ABclonal:Western blot - [KO Validated] NFKB1 Rabbit pAb (A6667) }

Western blot - [KO Validated] NFKB1 Rabbit pAb (A6667)

Western blot analysis of extracts from normal (control) and NFKB1 knockout (KO) HeLa cells, using NFKB1 antibody (A6667) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - [KO Validated] NFKB1 Rabbit pAb (A6667) }

Western blot - [KO Validated] NFKB1 Rabbit pAb (A6667)

Western blot analysis of extracts of HeLa cells, using [KO Validated] NFKB1 antibody (A6667) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - [KO Validated] NFKB1 Rabbit pAb (A6667) }

Immunohistochemistry - [KO Validated] NFKB1 Rabbit pAb (A6667)

Immunohistochemistry of paraffin-embedded mouse spleen using NFKB1 antibody (A6667) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] NFKB1 Rabbit pAb (A6667) }

Immunofluorescence - [KO Validated] NFKB1 Rabbit pAb (A6667)

Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] NFKB1 Rabbit pAb (A6667) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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