ABclonal

[KO Validated] NeuN Rabbit pAb

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Manufacture

ABclonal

Product name

[KO Validated] NeuN Rabbit pAb

Catalog No.

AB-A0951

Host species

Rabbit

Purification method

Affinity purification

Isotype

IgG

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NeuN (NP_001076044.1)

Gene ID

146713

Swiss prot

A6NFN3

Synonyms

FOX-3; FOX3; HRNBP3; NEUN; NeuN; RBFOX3

Calculated MW

33kDa/35kDa

Observed MW

50kDa

Reactivity

Human, Mouse, Rat

Tested applications

WB, IHC, IF

Recommended dilution

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.

Positive samples

Mouse brain, Rat brain

Customer validation

(Mouse brain, Rat brain)

IF(Mus musculus)

IHC(Mus musculus, Rattus norvegicus)

WB(Mus musculus, Rattus norvegicus)

PMID: 26758028  Title:SMAD4 is Involved in the Development of Endotoxin Tolerance in Microglia PMID: 30788732  Title:Mitochondrion-processed TERC regulates senescence without affecting telomerase activities.  PMID: 31874170  Title: A novel biscoumarin compound ameliorates cerebral ischemia reperfusion-induced mitochondrial oxidative injury via Nrf2/Keap1/ARE signaling  PMID: 32024024  Title: Synaptotagmin 1 Is Involved in Neuropathic Pain and Electroacupuncture-Mediated Analgesic Effect  PMID: 31029599  Title: The effect of lithium chloride on the attenuation of cognitive impairment in experimental hypoglycemic rats.  PMID: 32866269  Title: Reduced Dendritic Spines in the Visual Cortex Contralateral to the Optic Nerve Crush Eye in Adult Mice  PMID: 32871241  Title: Myocardial infarction-induced anxiety-like behavior is associated with epigenetic alterations in the hippocampus of rat  PMID: 33141045  Title: A novel biscoumarin derivative dephosphorylates ERK and alleviates apoptosis induced by mitochondrial oxidative damage in ischemic stroke mice  PMID: 33485120  Title: The new coumarin compound Bis 3 ameliorates cognitive disorder and suppresses brain-intestine-liver systematic oxidative stress in high-fat diet mice 
ABclonal:Western blot - [KO Validated] NeuN Rabbit pAb (A0951) }

Western blot - [KO Validated] NeuN Rabbit pAb (A0951)

Western blot analysis of extracts of various cell lines, using NeuN antibody (A0951) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunohistochemistry - [KO Validated] NeuN Rabbit pAb (A0951) }

Immunohistochemistry - [KO Validated] NeuN Rabbit pAb (A0951)

Immunohistochemistry of paraffin-embedded mouse brain using [KO Validated] NeuN Rabbit pAb (A0951) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] NeuN Rabbit pAb (A0951) }

Immunofluorescence - [KO Validated] NeuN Rabbit pAb (A0951)

Immunofluorescence analysis of rat brain using [KO Validated] NeuN Rabbit pAb (A0951) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] NeuN Rabbit pAb (A0951) }

Immunofluorescence - [KO Validated] NeuN Rabbit pAb (A0951)

Immunofluorescence analysis of mouse brain using [KO Validated] NeuN Rabbit pAb (A0951) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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