ABclonal

[KO Validated] N-Cadherin Rabbit mAb

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Manufacture

ABclonal

Product name

[KO Validated] N-Cadherin Rabbit mAb

Catalog No.

AB-A19083

Host species

Rabbit

Purification method

Affinity purification

Isotype

IgG

Immunogen

A synthesized peptide derived from human N Cadherin

Gene ID

1000

Swiss prot

P19022

Synonyms

CD325; CDHN; CDw325; NCAD; N Cadherin; CDH2

Calculated MW

140kDa

Observed MW

140KDa

Reactivity

Human, Mouse, Rat

Tested applications

WB, IHC, IF, 

Recommended dilution

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.

Positive samples

HeLa, 293T, NIH/3T3, C6

 
ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083) }

Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Western blot analysis of extracts from normal (control) and N-Cadherin knockout (KO) HeLa cells, using N-Cadherin antibody (A19083) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083) }

Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Western blot analysis of extracts of various cell lines, using N-Cadherin antibody (A19083) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

ABclonal:Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083) }

Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Immunohistochemistry of paraffin-embedded mouse heart using N-Cadherin Rabbit mAb (A19083) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083) }

Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Immunohistochemistry of paraffin-embedded rat heart using N-Cadherin Rabbit mAb (A19083) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] N-Cadherin Rabbit mAb (A19083) }

Immunofluorescence - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Immunofluorescence analysis of mouse heart using N-Cadherin Rabbit mAb (A19083) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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