ABclonal

[KO Validated] LC3B Rabbit pAb

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Manufacture

ABclonal

Product name

[KO Validated] LC3B Rabbit pAb

Catalog No.

AB-A7198

Host species

Rabbit

Purification method

Affinity purification

Isotype

IgG

Immunogen

A synthetic peptide of human LC3B

Gene ID

81631

Swiss prot

Q9GZQ8

Synonyms

LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3; MAP1LC3B; LC3A/LC3B

Calculated MW

14kDa

Observed MW

14kDa, 16kDa

Reactivity

Human, Mouse, Rat

Tested applications

WB, IHC, IF

Recommended dilution

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.

Positive samples

C6, HeLa, NIH/3T3, Pig liver

Customer validation

(MSC cells, Human lung cancer cell, C2C12, Squamous cell carcinoma, Mouse cells, 293T, U87MG, Rat cells)

WB(Mus musculus, Sus scrofa, Homo sapiens, Rattus norvegicus, Bombyx mori, Danio rerio)

IHC(Mus musculus, Homo sapiens)

IF(Homo sapiens)

IF(Mus musculus, Homo sapiens)

PMID: 27887947  Title: Berberine enhances the AMPK activation and autophagy and mitigates high glucose-induced apoptosis of mouse podocytes  PMID: 30683918  Title: The role of the LncRNA-FA2H-2-MLKL pathway in atherosclerosis by regulation of autophagy flux and inflammation through mTOR-dependent signaling.  PMID: 31109068  Title: Characteristics of the Life Cycle of Porcine Deltacoronavirus (PDCoV) In Vitro: Replication Kinetics, Cellular Ultrastructure and Virion Morphology, and Evidence of Inducing Autophagy PMID: 31437668   Title: Lysosomal dysfunction disturbs porcine oocyte maturation and developmental capacity by disorganizing chromosome/cytoskeleton and activating autophagy/apoptosis.  PMID: 31904151  Title: miR-133a-3p/FOXP3 axis regulates cell proliferation and autophagy in gastric cancer  PMID: 32001218  Title: Highly bioavailable berberine formulation ameliorates diabetic nephropathy through the inhibition of glomerular mesangial matrix expansion and the activation of autophagy PMID: 32398956  Title: Slit2/Robo1 Mitigates DSS-induced Ulcerative Colitis by Activating Autophagy in Intestinal Stem Cell  PMID: 32368828  Title: RNA m6 A methylation regulates sorafenib resistance in liver cancer through FOXO3-mediated autophagy  PMID: 32161543  Title: Ferulic Acid Attenuates Hypoxia/Reoxygenation Injury by Suppressing Mitophagy Through the PINK1/Parkin Signaling Pathway in H9c2 Cells  PMID: 31654713  Title: Steroid hormone 20-hydroxyecdysone induces the transcription and complex assembly of V-ATPases to facilitate autophagy in Bombyx mori  PMID: 31655340  Title: Macrophages-derived p38α promotes the experimental severe acute pancreatitis by regulating inflammation and autophagy  PMID: 32090563  Title: Neuroprotection by Walnut-Derived Peptides through Autophagy Promotion via Akt/mTOR Signaling Pathway against Oxidative Stress in PC12 Cells  PMID: 31827208   Title: Fundc1 is necessary for proper body axis formation during embryogenesis in zebrafish  PMID: 32420095  Title: Ginsenoside Rg3 protects heart against isoproterenol-induced myocardial infarction by activating AMPK mediated autophagy.  PMID: 30578841  Title: Corynoxine B ameliorates HMGB1-dependent autophagy dysfunction during manganese exposure in SH-SY5Y human neuroblastoma cells.  PMID: 33158440  Title: Deficiency of microglial Hv1 channel is associated with activation of autophagic pathway and ROS production in LPC-induced demyelination mouse model  PMID: 33316624  Title: The role of FOS-mediated autophagy activation in the indocyanine green-based photodynamic therapy for treating melanoma  PMID: 33294275  Title: MOB1A regulates glucose deprivation-induced autophagy via IL6-STAT3 pathway in gallbladder carcinoma  PMID: 33539420  Title: CircRNA ARFGEF1 functions as a ceRNA to promote oncogenic KSHV-encoded viral interferon regulatory factor induction of cell invasion and angiogenesis by upregulating glutaredoxin 3 
ABclonal:Western blot - [KO Validated] LC3B Rabbit pAb (A7198) }

Western blot - [KO Validated] LC3B Rabbit pAb (A7198)

Western blot analysis of extracts of various cell lines, using LC3B antibody (A7198) at 1:1000 dilution. C6 cells were treated by Chloroquine (50 μM) for 20 hours. Hela cells were treated by Chloroquine (50 μM) for 20 hours. NIH/3T3 cells were treated by Chloroquine (50 μM) for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] LC3B Rabbit pAb (A7198) }

Western blot - [KO Validated] LC3B Rabbit pAb (A7198)

Western blot analysis of extracts of pig liver, using LC3B antibody (A7198).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Western blot - [KO Validated] LC3B Rabbit pAb (A7198) }

Western blot - [KO Validated] LC3B Rabbit pAb (A7198)

Western blot analysis of extracts from normal (control) and LC3B knockout (KO) 293T cells, using LC3B antibody (A7198) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - [KO Validated] LC3B Rabbit pAb (A7198) }

Immunohistochemistry - [KO Validated] LC3B Rabbit pAb (A7198)

Immunohistochemistry of paraffin-embedded rat brain using LC3B antibody (A7198) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] LC3B Rabbit pAb (A7198) }

Immunohistochemistry - [KO Validated] LC3B Rabbit pAb (A7198)

Immunohistochemistry of paraffin-embedded human breast cancer using LC3B antibody (A7198) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] LC3B Rabbit pAb (A7198) }

Immunohistochemistry - [KO Validated] LC3B Rabbit pAb (A7198)

Immunohistochemistry of paraffin-embedded human stomach using LC3B antibody (A7198) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] LC3B Rabbit pAb (A7198) }

Immunofluorescence - [KO Validated] LC3B Rabbit pAb (A7198)

Immunofluorescence analysis of rat brain using LC3B antibody (A7198) at dilution of 1:100. Blue: DAPI for nuclear staining.
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