ABclonal

[KO Validated] FGF2 Rabbit mAb

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Manufacture

ABclonal

Product name

[KO Validated] FGF2 Rabbit mAb

Catalog No.

AB-A11488

Host species

Rabbit

Purification method

Affinity purification

Isotype

IgG

Immunogen

A synthesized peptide derived from human FGF2

Gene ID

2247

Swiss prot

P09038

Synonyms

BFGF; FGF-2; FGFB; HBGF-2

Calculated MW

31kDa

Observed MW

18KDa/22KDa/31KDa

Reactivity

Human, Mouse, Rat

Tested applications

WB, IHC, IF

Recommended dilution

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.

Positive samples

HeLa, SKOV3

Customer validation



ABclonal:Western blot - [KO Validated] FGF2 Rabbit mAb (A11488) }

Western blot - [KO Validated] FGF2 Rabbit mAb (A11488)

Western blot analysis of extracts from normal (control) and FGF2 knockout (KO) HeLa cells, using FGF2 Rabbit mAb (A11488) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - [KO Validated] FGF2 Rabbit mAb (A11488) }

Western blot - [KO Validated] FGF2 Rabbit mAb (A11488)

Western blot analysis of extracts of SKOV3 cells, using FGF2 Rabbit mAb (A11488) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - [KO Validated] FGF2 Rabbit mAb (A11488) }

Immunohistochemistry - [KO Validated] FGF2 Rabbit mAb (A11488)

Immunohistochemistry of paraffin-embedded human colon using [KO Validated] FGF2 Rabbit mAb (A11488) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] FGF2 Rabbit mAb (A11488) }

Immunohistochemistry - [KO Validated] FGF2 Rabbit mAb (A11488)

Immunohistochemistry of paraffin-embedded mouse brain using [KO Validated] FGF2 Rabbit mAb (A11488) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] FGF2 Rabbit mAb (A11488) }

Immunohistochemistry - [KO Validated] FGF2 Rabbit mAb (A11488)

Immunohistochemistry of paraffin-embedded rat brain using [KO Validated] FGF2 Rabbit mAb (A11488) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] FGF2 Rabbit mAb (A11488) }

Immunofluorescence - [KO Validated] FGF2 Rabbit mAb (A11488)

Immunofluorescence analysis of HeLa cells using FGF2 Rabbit mAb (A11488) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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