ABclonal

[KO Validated] ERK2 Rabbit pAb

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Product name [KO Validated] ERK2 Rabbit pAb
Catalog No. AB-A0229
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ERK2 (NP_620407.1).

Gene ID

5594

Swiss prot

P28482

Synonyms

ERK; ERK-2; ERK2; ERT1; MAPK2; P42MAPK; PRKM1; PRKM2; p38; p40; p41; p41mapk; p42-MAPK; MAPK1

Calculated MW

36kDa/41kDa

Observed MW

42KDa

Reactivity Human, Mouse, Rat
Tested applications WB IF
Recommended dilution WB 1:500 - 1:2000
IF 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples HeLa, A-549, NIH/3T3, C6, Mouse brain, Mouse thymus, Rat brain
Cellular location Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle
Customer validation

(Raw264.7, HeLa, LX2, Murine cells)

WB(Rattus norvegicus, Homo sapiens, Mus musculus)


PMID: 28534118  Title: Exercise-induced circulating extracellular vesicles protect against cardiac ischemia-reperfusion injury  PMID: 29018329  Title: CDDO and ATRA Instigate Differentiation of IMR32 Human Neuroblastoma Cells  PMID: 27966526  Title: Osteoblasts secrete Cxcl9 to regulate angiogenesis in bone  PMID: 29138800  Title: Broadleaf Mahonia attenuates granulomatous lobular mastitis?associated inflammation by inhibiting CCL?5 expression in macrophages  PMID: 29404859  Title: Serum Exosomes Attenuate H2O2-Induced Apoptosis in Rat H9C2 Cardiomyocytes via ERK1/2  PMID: 30240693  Title: Berberine modulates ASK1 signaling mediated through TLR4/TRAF2 via upregulation of miR-23a  PMID: 28789920  Title: The ω-carboxyl group of 7-ketocholesteryl-9-carboxynonanoate mediates the binding of oxLDL to CD36 receptor and enhances caveolin-1 expression in macrophages  PMID: 30782145  Title: Cathelicidin-related antimicrobial peptide protects against myocardial ischemia/reperfusion injury.  PMID: 31275851  Title: TICRR Contributes to Tumorigenesis Through Accelerating DNA Replication in Cancers.  PMID: 31247479  Title: Regulation of an osteon-like concentric microgrooved surface on osteogenesis and osteoclastogenesis.  PMID: 32270801  Title: Molecular mechanisms of selenium-biofortified soybean protein and polyphenol conjugates in protecting mouse skin damaged by UV-B  PMID: 31900224  Title: Dopamine D1 receptor-mediated activation of the ERK signaling pathway is involved in the osteogenic differentiation of bone mesenchymal stem cells  PMID: 31900224  Title: Dopamine D1 receptor-mediated activation of the ERK signaling pathway is involved in the osteogenic differentiation of bone mesenchymal stem cells  PMID: 31511502  Title: SNRPB promotes the tumorigenic potential of NSCLC in part by regulating RAB26.  PMID: 27333037  Title: Enho Mutations Causing Low Adropin: A Possible Pathomechanism of MPO-ANCA Associated Lung Injury.  PMID: 32565723  Title: The Effects of Sidt2 on the Inflammatory Pathway in Mouse Mesangial Cells  PMID: 32764906  Title: Increased Osteoblastic Cxcl9 Contributes to the Uncoupled Bone Formation and Resorption in Postmenopausal Osteoporosis  PMID: 32876895   Title: MicroRNA-532-3p Regulates Pro-Inflammatory Human THP-1 Macrophages by Targeting ASK1/p38 MAPK Pathway  PMID: 33400013  Title:  The effect of Q-switched 1064-nm dymium-doped yttrium aluminum garnet laser on the skin barrier and collagen synthesis through miR-24-3p 

 

ABclonal:Western blot - [KO Validated] ERK2 Rabbit pAb (A0229) }

Western blot - [KO Validated] ERK2 Rabbit pAb (A0229)

Western blot analysis of extracts of various cell lines, using ERK2 antibody (A0229) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] ERK2 Rabbit pAb (A0229) }

Western blot - [KO Validated] ERK2 Rabbit pAb (A0229)

Western blot analysis of extracts from normal (control) and ERK2 knockout (KO) HeLa cells, using ERK2 antibody (A0229) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
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