ABclonal

[KO Validated] Cytochrome c Rabbit pAb

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Manufacture

ABclonal

Product name

[KO Validated] Cytochrome c Rabbit pAb

Catalog No.

AB-A13430

Host species

Rabbit

Purification method

Affinity purification

Isotype

IgG

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human Cytochrome c (NP_061820.1)

Gene ID

54205

Swiss prot

P99999

Synonyms

CYCS; CYC; HCS; THC4; cytochrome c

Calculated MW

11kDa

Observed MW

11kDa

Reactivity

Human, Mouse, Rat

Tested applications

WB, IHC, IF

Recommended dilution

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.

Positive samples

Raji, HeLa, Mouse lung, RAW264.7, Rat testis, Rat lung, 293T

Customer validation

WB(Sanghuangporus vaninii, Mus musculus, Rattus norvegicus)

PMID: 29956756  Title: ZIC1 acts a tumor suppressor in breast cancer by targeting survivin.  PMID: 32368187  Title: Roles of eIF3m in the tumorigenesis of triple negative breast cancer  PMID: 32096479  Title: Adipose-derived mesenchymal stem cells-derived exosome-mediated microRNA-342-5p protects endothelial cells against atherosclerosis  PMID: 31466050  Title: Genipin attenuates mitochondrial-dependent apoptosis, endoplasmic reticulum stress, and inflammation via the PI3K/AKT pathway in acute lung injury.  PMID: 32042730  Title: The atheroprotective roles of heart-protecting musk pills against atherosclerosis development in apolipoprotein E-deficient mice  PMID: 30913525  Title: Synthesis and anti-cancer evaluation of folic acid-peptide- paclitaxel conjugates for addressing drug resistance.  PMID: 30532520  Title: Arsenic sulfide amplifies JQ1 toxicity via mitochondrial pathway in gastric and colon cancer cells.  PMID: 31817556  Title: Chinese Giant Salamander (Andrias davidianus) Iridovirus Infection Leads to Apoptotic Cell Death through Mitochondrial Damage, Caspases Activation, and Expression of Apoptotic-Related Genes.  PMID: 32589884  Title: 6H2L, a novel synthetic derivative of bifendate, induces apoptosis in hepatoma cells via mitochondrial and MAPK pathway  PMID: 32698821  Title: Electroacupuncture alleviates polycystic ovary syndrome-like symptoms through improving insulin resistance, mitochondrial dysfunction, and endoplasmic reticulum stress via enhancing autophagy in rats  PMID: 33109277  Title: Novel mechanisms underlying anti-polycystic ovary like syndrome effects of electroacupuncture in rats: suppressing SREBP1 to mitigate insulin resistance,mitochondrial dysfunction and oxidative stress  PMID: 33130480  Title: Mitophagy–lysosomal pathway is involved in silver nanoparticle-induced apoptosis in A549 cell PMID: 32842537  Title: Giardia duodenalis Induces Apoptosis in Intestinal Epithelial Cells via Reactive Oxygen Species-Mediated Mitochondrial Pathway In Vitro  PMID: 32619556  Title: Glycolysis inhibition and apoptosis induction in human prostate cancer cells by FV-429-mediated regulation of AR-AKT-HK2 signaling network  PMID: 33603340  Title: Loganin Attenuates Septic Acute Renal Injury with the Participation of AKT and Nrf2/HO-1 Signaling Pathways 
ABclonal:Western blot - [KO Validated] Cytochrome c Rabbit pAb (A13430) }

Western blot - [KO Validated] Cytochrome c Rabbit pAb (A13430)

Western blot analysis of extracts of various cell lines, using Cytochrome c Rabbit pAb (A13430) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - [KO Validated] Cytochrome c Rabbit pAb (A13430) }

Western blot - [KO Validated] Cytochrome c Rabbit pAb (A13430)

Western blot analysis of extracts from normal (control) and Cytochrome c knockout (KO) 293T cells, using Cytochrome c antibody (A13430) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - [KO Validated] Cytochrome c Rabbit pAb (A13430) }

Immunohistochemistry - [KO Validated] Cytochrome c Rabbit pAb (A13430)

Immunohistochemistry of paraffin-embedded rat lung using Cytochrome c antibody (A13430) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] Cytochrome c Rabbit pAb (A13430) }

Immunohistochemistry - [KO Validated] Cytochrome c Rabbit pAb (A13430)

Immunohistochemistry of paraffin-embedded human tonsil using Cytochrome c antibody (A13430) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] Cytochrome c Rabbit pAb (A13430) }

Immunohistochemistry - [KO Validated] Cytochrome c Rabbit pAb (A13430)

Immunohistochemistry of paraffin-embedded human liver cancer using Cytochrome c antibody (A13430) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] Cytochrome c Rabbit pAb (A13430) }

Immunohistochemistry - [KO Validated] Cytochrome c Rabbit pAb (A13430)

Immunohistochemistry of paraffin-embedded mouse kidney using Cytochrome c antibody (A13430) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] Cytochrome c Rabbit pAb (A13430) }

Immunofluorescence - [KO Validated] Cytochrome c Rabbit pAb (A13430)

Immunofluorescence analysis of HeLa cells using Cytochrome c antibody (A13430) at dilution of 1:100. Blue: DAPI for nuclear staining.
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