ABclonal

[KO Validated] CSPG4 Rabbit pAb

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Manufacture

ABclonal

Product name

[KO Validated] CSPG4 Rabbit pAb

Catalog No.

AB-A3592

Host species

Rabbit

Purification method

Affinity purification

Isotype

IgG

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1950-2210 of human CSPG4 (NP_001888.2)

Gene ID

1464

Swiss prot

Q6UVK1

Synonyms

CSPG4; HMW-MAA; MCSP; MCSPG; MEL-CSPG; MSK16; NG2

Calculated MW

250kDa

Observed MW

270kDa

Reactivity

Human, Mouse, Rat

Tested applications

WB, IHC, IF

Recommended dilution

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.

Positive samples

HeLa

Customer validation

WB(Mus musculus)

IF(Mus musculus)

IF(Mus musculus)


PMID: 32581380  Title: Reversing a Model of Parkinson's Disease With in Situ Converted Nigral Neurons PMID: 32920091  Title: Intermittent caloric restriction with a modified fasting-mimicking diet ameliorates autoimmunity and promotes recovery in a mouse model of multiple sclerosis 
ABclonal:Western blot - [KO Validated] CSPG4 Rabbit pAb (A3592) }

Western blot - [KO Validated] CSPG4 Rabbit pAb (A3592)

Western blot analysis of extracts from normal (control) and CSPG4 knockout (KO) HeLa cells, using CSPG4 antibody (A3592) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] CSPG4 Rabbit pAb (A3592) }

Western blot - [KO Validated] CSPG4 Rabbit pAb (A3592)

Western blot analysis of extracts of HeLa cells, using CSPG4 antibody (A3592) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
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