ABclonal

[KO Validated] CREB1 Rabbit mAb

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Manufacture

ABclonal

Product name

[KO Validated] CREB1 Rabbit mAb

Catalog No.

AB-A10826

Host species

Rabbit

Purification method

Affinity purification

Isotype

IgG

Immunogen

A10826

Gene ID

1385

Swiss prot

P16220

Synonyms

CREB1; CREB; CREB-1

Calculated MW

25kDa/35kDa/36kDa

Observed MW

40kDa

Reactivity

Human, Mouse, Rat

Tested applications

WB

Recommended dilution

WB 1:500 - 1:2000

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.

Positive samples

293T, Mouse lung, Mouse brain, Rat brain

Customer validation

WB(Homo sapiens,Mus musculus)

IF(Homo sapiens,Mus musculus)

IP(Homo sapiens,Mus musculus)

IF(Mus musculus)

PMID: 31418947  Title: Inhibition of CREB-mediated ZO-1 and Activation of NF-κB-induced IL-6 by Colonic Epithelial MCT4 Destroys Intestinal Barrier Function.  PMID: 33040080  Title: USP7 mediates pathological hepatic de novo lipogenesis through promoting stabilization and transcription of ZNF638  PMID: 33067662  Title: CGRP-Mediated Prolactin Upregulation: a Possible Pathomechanism in IgG4-Related Disease 
ABclonal:Western blot - [KO Validated] CREB1 Rabbit mAb (A10826) }

Western blot - [KO Validated] CREB1 Rabbit mAb (A10826)

Western blot analysis of extracts of various cell lines, using CREB1 antibody (A10826) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] CREB1 Rabbit mAb (A10826) }

Western blot - [KO Validated] CREB1 Rabbit mAb (A10826)

Western blot analysis of extracts from normal (control) and CREB1 knockout (KO) 293T cells, using CREB1 antibody (A10826) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
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