ABclonal

[KO Validated] Caspase-9 Rabbit pAb

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Manufacture

ABclonal

Product name

[KO Validated] Caspase-9 Rabbit pAb

Catalog No.

AB-A0281

Host species

Rabbit

Purification method

Affinity purification

Isotype

IgG

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-98 of human Caspase-9 (NP_001220.2)

Gene ID

842

Swiss prot

P55211

Synonyms

CASP9; APAF-3; APAF3; ICE-LAP6; MCH6; PPP1R56; caspase-9; casp9; Caspase 9

Calculated MW

17kDa/30kDa/36kDa/46kDa

Observed MW

36KDa/41KDa/46KDa

Reactivity

Human, Mouse, Rat

Tested applications

WB, IHC, IF

Recommended dilution

WB 1:1000 - 1:50000
IHC 1:50 - 1:1000
IF 1:50 - 1:1000
IP 1:20 - 1:50

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.

Positive samples

HeLa, Jurkat, Mouse brain, Rat brain

Customer validation

 

WB(Homo sapiens, Mus musculus, Bos taurus, Sanghuangporus vaninii)
1. PMID: 32301534  Title:  SIRT5 impairs aggregation and activation of the signaling adaptor MAVS through catalyzing lysine desuccinylation  2. PMID: 32950971  Title: GPA peptide inhibits NLRP3 inflammasome activation to ameliorate colitis through AMPK pathway 
ABclonal:Western blot - [KO Validated] Caspase-9 Rabbit pAb (A0281) }

Western blot - [KO Validated] Caspase-9 Rabbit pAb (A0281)

Western blot analysis of extracts of various cell lines, using Caspase-9 pAb (A0281) at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - [KO Validated] Caspase-9 Rabbit pAb (A0281) }

Western blot - [KO Validated] Caspase-9 Rabbit pAb (A0281)

Western blot analysis of extracts from normal (control) and Caspase-9 knockout (KO) HeLa cells, using Caspase-9 antibody (A0281) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - [KO Validated] Caspase-9 Rabbit pAb (A0281) }

Immunohistochemistry - [KO Validated] Caspase-9 Rabbit pAb (A0281)

Immunohistochemistry of paraffin-embedded human placenta using Caspase-9 antibody (A0281) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] Caspase-9 Rabbit pAb (A0281) }

Immunofluorescence - [KO Validated] Caspase-9 Rabbit pAb (A0281)

Immunofluorescence analysis of C6 cells using Caspase-9 antibody (A0281) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] Caspase-9 Rabbit pAb (A0281) }

Immunofluorescence - [KO Validated] Caspase-9 Rabbit pAb (A0281)

Immunofluorescence analysis of NIH/3T3 cells using Caspase-9 antibody (A0281) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] Caspase-9 Rabbit pAb (A0281) }

Immunofluorescence - [KO Validated] Caspase-9 Rabbit pAb (A0281)

Immunofluorescence analysis of U-2 OS cells using Caspase-9 antibody (A0281) at dilution of 1:100. Blue: DAPI for nuclear staining.
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