ABclonal

[KO Validated] Bax Rabbit pAb

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Product name [KO Validated] Bax Rabbit pAb
Catalog No. AB-A0207
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bax (NP_620116.1).

Gene ID

581

Swiss prot

Q07812

Synonyms

BCL2L4; BAX

Calculated MW

4kDa/12kDa/15kDa/18kDa/19kDa/21kDa/24kDa

Observed MW

21KDa

Reactivity Human, Mouse, Rat
Tested applications WB IHC IF IP
Recommended dilution WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples HT-1080, Raji, 293T
Cellular location Cytoplasm, Cytoplasm, Mitochondrion membrane, Single-pass membrane protein
Customer validation

(Eoma cell, Human liver cell, Human pancreatic cancer cells, Human skin T cell lymphoma, Hut102, HCT116)

WB(Homo sapiens, Rattus norvegicus, Mus musculus, Gallus gallus, Capra hircus, Ctenopharyngodon, Ctenopharyngodon idellus, Giardia duodenalis)

IF(Homo sapiens)

75 publications [link] PMID: 24133586  Title: Systematic screen with kinases inhibitors reveals kinases play distinct roles in growth of osteoprogenitor cells  PMID: 24427328  Title: KAT5 silencing induces apoptosis of GBC-SD cells through p38MAPK-mediated upregulation of cleaved Casp9  PMID: 28534118  Title: Exercise-induced circulating extracellular vesicles protect against cardiac ischemia-reperfusion injury  PMID: 29033244  Title: iASPP Is an Antioxidative Factor and Drives Cancer Growth and Drug Resistance by Competing with Nrf2 for Keap1 Binding  PMID: 29404859  Title: Serum Exosomes Attenuate H2O2-Induced Apoptosis in Rat H9C2 Cardiomyocytes via ERK1/2  PMID: 29412164  Title: SGK1 inhibits PM2. 5-induced apoptosis and oxidative stress in human lung alveolar epithelial A549 cells  PMID: 29510730  Title: The lncRNA MACC1-AS1 promotes gastric cancer cell metabolic plasticity via AMPK/Lin28 mediated mRNA stability of MACC1  PMID: 29736179  Title: Natural Compound Methyl Protodioscin Suppresses Proliferation and Inhibits Glycolysis in Pancreatic Cancer  PMID: 29743526  Title:  Pretreatment of Huaiqihuang extractum protects against cisplatin-induced nephrotoxicity  PMID: 29740735  Title: Dandelion root extract protects NCM460 colonic cells and relieves experimental mouse colitis 
ABclonal:Western blot - [KO Validated] Bax Rabbit pAb (A0207) }

Western blot - [KO Validated] Bax Rabbit pAb (A0207)

Western blot analysis of extracts from normal (control) and Bax knockout (KO) 293T cells, using Bax antibody (A0207) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Western blot - [KO Validated] Bax Rabbit pAb (A0207) }

Western blot - [KO Validated] Bax Rabbit pAb (A0207)

Western blot analysis of extracts of various cell lines, using Bax antibody (A0207) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - [KO Validated] Bax Rabbit pAb (A0207) }

Immunohistochemistry - [KO Validated] Bax Rabbit pAb (A0207)

Immunohistochemistry of paraffin-embedded human esophageal cancer using [KO Validated] Bax Rabbit pAb (A0207) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] Bax Rabbit pAb (A0207) }

Immunofluorescence - [KO Validated] Bax Rabbit pAb (A0207)

Immunofluorescence analysis of NIH/3T3 cells using Bax antibody (A0207) at dilution of 1:100. Blue: DAPI for nuclear staining.

 

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