ABclonal

[KO Validated] APP Rabbit mAb

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Manufacture

ABclonal

Product name

[KO Validated] APP Rabbit mAb

Catalog No.

AB-A17911

Host species

Rabbit

Purification method

Affinity purification

Isotype

IgG

Immunogen

A synthesized peptide derived from human APP

Gene ID

351

Swiss prot

P05067

Synonyms

AAA; ABETA; ABPP; AD1; APPI; CTFgamma; CVAP; PN-II; PN2; Amyloid beta A4; APP; Aβ42; preA4

Calculated MW

95kDa

Observed MW

100-140kDa

Reactivity

Human, Mouse, Rat

Tested applications

WB, IHC, IF, IP

Recommended dilution

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.

Positive samples

HeLa, U-87MG, Mouse brain, Rat brain, 293T

Customer validation

 

ABclonal:Western blot - [KO Validated] APP Rabbit mAb (A17911) }

Western blot - [KO Validated] APP Rabbit mAb (A17911)

Western blot analysis of extracts from normal (control) and APP knockout (KO) 293T cells, using APP antibody (A17911) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] APP Rabbit mAb (A17911) }

Western blot - [KO Validated] APP Rabbit mAb (A17911)

Western blot analysis of extracts of various cell lines, using APP antibody (A17911) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KO Validated] APP Rabbit mAb (A17911) }

Immunohistochemistry - [KO Validated] APP Rabbit mAb (A17911)

Immunohistochemistry of paraffin-embedded mouse brain using APP Rabbit mAb (A17911) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] APP Rabbit mAb (A17911) }

Immunohistochemistry - [KO Validated] APP Rabbit mAb (A17911)

Immunohistochemistry of paraffin-embedded rat brain using APP Rabbit mAb (A17911) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] APP Rabbit mAb (A17911) }

Immunohistochemistry - [KO Validated] APP Rabbit mAb (A17911)

Immunohistochemistry of paraffin-embedded human brain using APP Rabbit mAb (A17911) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] APP Rabbit mAb (A17911) }

Immunofluorescence - [KO Validated] APP Rabbit mAb (A17911)

Immunofluorescence analysis of HeLa cells using APP Rabbit mAb (A17911) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] APP Rabbit mAb (A17911) }

Immunoprecipitation - [KO Validated] APP Rabbit mAb (A17911)

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug APP antibody (A17911). Western blot was performed from the immunoprecipitate using APP antibody (A17911) at a dilition of 1:1000.
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