ABclonal

[KO Validated] active + pro Caspase-3 Rabbit mAb

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Manufacture

ABclonal

Product name

[KO Validated] active + pro Caspase-3 Rabbit mAb

Catalog No.

AB-A19654

Host species

Rabbit

Purification method

Affinity purification

Isotype

IgG

Immunogen

A synthesized peptide derived from human active + pro Caspase-3

Gene ID

836

Swiss prot

P42574

Synonyms

CPP32; CPP32B; SCA-1; Active Caspase 3; CASP3; active Caspase-3; Caspase 3; Caspase-3 p12; caspase-3

Calculated MW

17kDa/32kDa

Observed MW

17KDa/32kDa/35KDa

Reactivity

Human, Mouse, Rat

Tested applications

WB, IF

Recommended dilution

WB 1:500 - 1:2000
IF 1:50 - 1:200

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.

Positive samples

Jurkat, Mouse lung, Mouse liver, NIH/3T3, Rat spleen, Rat liver, 293T

Customer validation

WB(Homo sapiens, Sanghuangporus vaninii, Mus musculus, Giardia duodenalis, Rattus norvegicus)

PMID: 32525021  Title: Giardia duodenalis induces extrinsic pathway of apoptosis in intestinal epithelial cells through activation of TNFR1 and K63 de-ubiquitination of RIP1 in vitro 
PMID: 32629056  Title: Characterization of a polysaccharide from Sanghuangporus vaninii and its antitumor regulation via activation of the p53 signaling pathway in breast cancer MCF-7 …  PMID: 33325535  Title: Knockdown of long non‑coding RNA ANRIL inhibits the proliferation and promotes the apoptosis of Burkitt lymphoma cells through the TGF‑β1 signaling pathway  PMID: 32211324  Title: LOXL2 Expression Status Is Correlated With Molecular Characterizations of Cervical Carcinoma and Associated With Poor Cancer Survival via Epithelial-Mesenchymal Transition (EMT) Phenotype  PMID: 32016695  Title: Rutin protects against pirarubicin-induced cardiotoxicity by adjusting microRNA-125b-1-3p-mediated JunD signaling pathway  PMID: 32842537  Title: Giardia duodenalis Induces Apoptosis in Intestinal Epithelial Cells via Reactive Oxygen Species-Mediated Mitochondrial Pathway In Vitro  PMID: 32928312  Title: Palmatine ameliorates high fat diet induced impaired glucose tolerance 
ABclonal:Western blot - [KO Validated] active + pro Caspase-3 Rabbit mAb (A19654) }

Western blot - [KO Validated] active + pro Caspase-3 Rabbit mAb (A19654)

Western blot analysis of extracts from normal (control) and active + pro Caspase-3 knockout (KO) 293T cells, using active + pro Caspase-3 antibody (A19654) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - [KO Validated] active + pro Caspase-3 Rabbit mAb (A19654) }

Western blot - [KO Validated] active + pro Caspase-3 Rabbit mAb (A19654)

Western blot analysis of extracts of various cell lines, using active + pro Caspase-3 antibody (A19654) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - [KO Validated] active + pro Caspase-3 Rabbit mAb (A19654) }

Western blot - [KO Validated] active + pro Caspase-3 Rabbit mAb (A19654)

Western blot analysis of extracts of various cell lines, using active + pro Caspase-3 antibody (A19654) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Western blot - [KO Validated] active + pro Caspase-3 Rabbit mAb (A19654) }

Western blot - [KO Validated] active + pro Caspase-3 Rabbit mAb (A19654)

Western blot analysis of extracts of Jurkat cells, using active + pro Caspase 3 antibody (A19654) at 1:1000 dilution.Jurkat cells were treated by Staurosporine(1uM) at room temperature for 3 hours .
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - [KO Validated] active + pro Caspase-3 Rabbit mAb (A19654) }

Immunofluorescence - [KO Validated] active + pro Caspase-3 Rabbit mAb (A19654)

Immunofluorescence analysis of HeLa cells using active + pro Caspase-3 antibody (A19654).HeLa cells were treated by Etoposide (25 uM) at 37℃ for 5 hours. Blue: DAPI for nuclear staining.

 

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