ABclonal

[KO Validated] β-Catenin Rabbit mAb

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Manufacture

ABclonal

Product name

[KO Validated] β-Catenin Rabbit mAb

Catalog No.

AB-A19657

Host species

Rabbit

Purification method

Affinity purification

Isotype

IgG

Immunogen

A synthesized peptide derived from human beta Catenin.

Gene ID

1499

Swiss prot

P35222

Synonyms

CTNNB; MRD19; armadillo; beta Catenin; CTNNB1

Calculated MW

92kDa

Observed MW

92kDa

Reactivity

Human, Mouse, Rat

Tested applications

WB, IHC, IF

Recommended dilution

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.

Positive samples

HeLa, Mouse brain, 293T

Customer validation

 WB(Homo sapiens)


PMID: 33014112  Title: Role of Chrysophanol in Epithelial-Mesenchymal Transition in Oral Cancer Cell Lines via a Wnt-3-Dependent Pathway 
ABclonal:Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657) }

Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)

Western blot analysis of extracts of HeLa cells, using β-Catenin antibody (A19657) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657) }

Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)

Western blot analysis of extracts of Mouse brain, using β-Catenin antibody (A19657) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657) }

Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)

Western blot analysis of extracts from normal (control) and β-Catenin knockout (KO) 293T cells, using β-Catenin antibody (A19657) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657) }

Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunohistochemistry of paraffin-embedded Human cervical squamous cell carcinoma using [KO Validated] β-Catenin Rabbit mAb (A19657) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657) }

Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunohistochemistry of paraffin-embedded Human Invasive ductal carcinoma of breast using [KO Validated] β-Catenin Rabbit mAb (A19657) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657) }

Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunohistochemistry of paraffin-embedded human lung squamous carcinoma tissue using [KO Validated] β-Catenin Rabbit mAb (A19657) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657) }

Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunohistochemistry of paraffin-embedded Human pancreatic ductal carcinoma using [KO Validated] β-Catenin Rabbit mAb (A19657) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] β-Catenin Rabbit mAb (A19657) }

Immunofluorescence - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunofluorescence analysis of C6 cells using β-Catenin antibody (A19657) at dilution of 1:100. Blue: DAPI for nuclear staining.
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