ABclonal

JNK1 Rabbit pAb

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Product name JNK1 Rabbit pAb
Catalog No. AB- A0288
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 245-345 of human JNK1 (NP_620635.1).

Gene ID

5599

Swiss prot

P45983

Synonyms

MAPK8; JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2; PRKM8; SAPK1; SAPK1c

Calculated MW

35kDa/44kDa/48kDa

Observed MW

44KDa

Reactivity Human, Mouse, Rat
Tested applications WB IHC IF
Recommended dilution WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples SH-SY5Y, Mouse kidney, Rat heart
Cellular location Cytoplasm, Nucleus
Customer validation

(Mouse heart tissue)

WB(Homo sapiens, Rattus norvegicus, Mus musculus)

IF(Mus musculus)

IHC(Mus musculus)


PMID: 26456053  Title: Novel role of TRPV2 in promoting the cytotoxicity of H2O2-mediated oxidative stress in human hepatoma cells  PMID: 26603550  Title: Ganoderma lucidum polysaccharide peptide prevents renal ischemia reperfusion injury via counteracting oxidative stress  PMID: 28680855  Title: p21-Activated Kinase 4 Signaling Promotes Japanese Encephalitis Virus-Mediated Inflammation in Astrocytes  PMID: 28789920  Title: The ω-carboxyl group of 7-ketocholesteryl-9-carboxynonanoate mediates the binding of oxLDL to CD36 receptor and enhances caveolin-1 expression in macrophages  PMID: 1467414   Title: Essential roles of S100A10 in Toll-like receptor signaling and immunity to infection.  PMID: 32116720  Title: Alleviation of Synovial Inflammation of Juanbi-Tang on Collagen-Induced Arthritis and TNF-Tg Mice Model  PMID: 31247479  Title: Regulation of an osteon-like concentric microgrooved surface on osteogenesis and osteoclastogenesis.  PMID: 32270801  Title: Molecular mechanisms of selenium-biofortified soybean protein and polyphenol conjugates in protecting mouse skin damaged by UV-B  PMID: 31900224  Title: Dopamine D1 receptor-mediated activation of the ERK signaling pathway is involved in the osteogenic differentiation of bone mesenchymal stem cells  PMID: 31467414   Title: Essential roles of S100A10 in Toll-like receptor signaling and immunity to infection.  ......

 

ABclonal:Western blot - JNK1 Rabbit pAb (A0288) }

Western blot - JNK1 Rabbit pAb (A0288)

Western blot analysis of extracts of SH-SY5Y cells, using JNK1 antibody (A0288) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - JNK1 Rabbit pAb (A0288) }

Western blot - JNK1 Rabbit pAb (A0288)

Western blot analysis of extracts of various cell lines, using JNK1 antibody (A0288) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - JNK1 Rabbit pAb (A0288) }

Immunohistochemistry - JNK1 Rabbit pAb (A0288)

Immunohistochemistry of paraffin-embedded rat pancreas using JNK1 Rabbit pAb (A0288) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - JNK1 Rabbit pAb (A0288) }

Immunohistochemistry - JNK1 Rabbit pAb (A0288)

Immunohistochemistry of paraffin-embedded human thyroid cancer using JNK1 Rabbit pAb (A0288) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - JNK1 Rabbit pAb (A0288) }

Immunofluorescence - JNK1 Rabbit pAb (A0288)

Immunofluorescence analysis of HeLa cells using JNK1 antibody (A0288) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

 

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