ABclonal

Insulin Receptor Rabbit pAb

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Product name Insulin Receptor Rabbit pAb
Catalog No. AB- A0287
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1130-1230 of human Insulin Receptor (NP_000199.2).

Gene ID

3643

Swiss prot

P06213

Synonyms

CD220; HHF5; INSR

Calculated MW

155kDa/156kDa

Observed MW

180kDa

Reactivity Mouse, Rat
Tested applications WB IHC
Recommended dilution WB 1:500 - 1:2000
IHC 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples Mouse liver, Rat liver
Cellular location Cell membrane, Single-pass type I membrane protein
Customer validation

WB(Mus musculus)


PMID: 29873092  Title: Abnormal gene methylation during embryonic development after preimplantation genetic testing increases risk of liver‐derived insulin resistance  ......

 

 

ABclonal:Western blot - Insulin Receptor Rabbit pAb (A0287) }

Western blot - Insulin Receptor Rabbit pAb (A0287)

Western blot analysis of extracts of various cell lines, using Insulin Receptor antibody (A0287) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
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