ABclonal

iNOS Rabbit pAb

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Product name iNOS Rabbit pAb
Catalog No. AB- A0312
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 500-730 of human iNOS (NP_000616.3).

Gene ID

4843

Swiss prot

P35228

Synonyms

NOS; INOS; NOS2A; HEP-NOS; iNOS; NOS2

Calculated MW

126kDa/131kDa

Observed MW

130KDa

Reactivity Human, Mouse, Rat
Tested applications WB IHC
Recommended dilution WB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples RAW264.7
Cellular location
Customer validation

(Mouse embryonal fibroblast cell, Cell Line of African Green Monkey Renal Fibroblasts Transformed by SV40 Virus Gene)

ELISA(Mus musculus)

WB(Mus musculus)

WB(Mus musculus, Rattus norvegicus, Trifolium pratense, Schisandra chinensis, Ctenopharyngodon idellus, R. hotaoense)


PMID: 29391237  Title: Sulforaphane attenuates microglia-mediated neuronal necroptosis through down-regulation of MAPK/NF-κB signaling pathways in LPS-activated BV-2 microglia  PMID: 30946805  Title: Dauricine negatively regulates lipopolysaccharide- or cecal ligation and puncture-induced inflammatory response via NF-κB inactivation.  PMID: 31030094  Title: Stemona alkaloids suppress the positive feedback loop between M2 polarization and fibroblast differentiation by inhibiting JAK2/STAT3 pathway in fibroblasts and CXCR4/PI3K/AKT1 pathway in macrophages.  PMID: 31549780  Title: Chronic sleep fragmentation shares similar pathogenesis with neurodegenerative diseases: Endosome-autophagosome-lysosome pathway dysfunction and microglia-mediated neuroinflammation.  PMID: 31870966  Title: DDAH-1 via HIF-1 target genes improves cerebral ischemic tolerance after hypoxic preconditioning and middle cerebral artery occlusion-reperfusion  PMID: 32326385  Title: Anti-Inflammatory and Antioxidant Effects of Anthocyanins of Trifolium pratense (Red Clover) in Lipopolysaccharide-Stimulated RAW-267.4 Macrophages  PMID: 31626784  Title: Fingolimod promotes angiogenesis and attenuates ischemic brain damage via modulating microglial polarization  PMID: 32278833  Title: MiR-144-5p limits experimental abdominal aortic aneurysm formation by mitigating M1 macrophage-associated inflammation: Suppression of TLR2 and OLR1  PMID: 32186565  Title: Investigation of the active components and mechanisms of Schisandra chinensis in the treatment of asthma based on a network pharmacology approach and experimental validation  PMID: 31888180  Title: Inducible Nitric Oxide Synthase (iNOS) Mediates Vascular Endothelial Cell Apoptosis in Grass Carp Reovirus (GCRV)-Induced Hemorrhage.  ......
ABclonal:Western blot - iNOS Rabbit pAb (A0312) }

Western blot - iNOS Rabbit pAb (A0312)

Western blot analysis of extracts of RAW264.7 cells, using iNOS antibody (A0312) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - iNOS Rabbit pAb (A0312) }

Immunohistochemistry - iNOS Rabbit pAb (A0312)

Immunohistochemistry of paraffin-embedded rat lung using iNOS Rabbit pAb (A0312) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - iNOS Rabbit pAb (A0312) }

Immunohistochemistry - iNOS Rabbit pAb (A0312)

Immunohistochemistry of paraffin-embedded human esophageal cancer using iNOS Rabbit pAb (A0312) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - iNOS Rabbit pAb (A0312) }

Immunohistochemistry - iNOS Rabbit pAb (A0312)

Immunohistochemistry of paraffin-embedded mouse spleen using iNOS Rabbit pAb (A0312) at dilution of 1:100 (40x lens).
 
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