ABclonal

IGF1 Rabbit pAb

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Product name IGF1 Rabbit pAb
Catalog No. AB- A0303
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 90 to the C-terminus of human IGF1 (NP_000609.1).

Gene ID

3479

Swiss prot

P05019

Synonyms

IGF1; IGF-I; IGFI; MGF

Calculated MW

15kDa/17kDa/21kDa

Observed MW

18KDa

Reactivity Human, Mouse, Rat
Tested applications WB IHC
Recommended dilution WB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples HeLa, Mouse testis
Cellular location Secreted
Customer validation

(H9c2)



ABclonal:Western blot - IGF1 Rabbit pAb (A0303) }

Western blot - IGF1 Rabbit pAb (A0303)

Western blot analysis of extracts of various cell lines, using IGF1 Rabbit pAb (A0303) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - IGF1 Rabbit pAb (A0303) }

Immunohistochemistry - IGF1 Rabbit pAb (A0303)

Immunohistochemistry of paraffin-embedded human lung cancer using IGF1 Rabbit pAb (A0303) at dilution of 1:100 (40x lens).  
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