ABclonal

Iba1 Rabbit pAb

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  • €59,00
  • Regular price €101,00
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Manufacture

ABclonal

Product name

Iba1 Rabbit pAb

Catalog No.

AB-A12391

Host species

Rabbit

Purification method

Affinity purification

Isotype

IgG

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human Iba1 (NP_001614.3).

Gene ID

199

Swiss prot

P55008

Synonyms

AIF1; AIF-1; IBA1; IRT-1; IRT1; Iba1

Calculated MW

10kDa/14kDa/16kDa

Observed MW

15kDa

Reactivity

Human, Mouse, Rat

Tested applications

WB, IHC, IF

Recommended dilution

WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.

Positive samples

THP-1, Mouse spleen, Rat testis, Rat spleen

Customer validation

WB(Mus musculus)


PMID: 32871241 

Title: Myocardial infarction-induced anxiety-like behavior is associated with epigenetic alterations in the hippocampus of rat 
ABclonal:Western blot - Iba1 Rabbit pAb (A12391) }

Western blot - Iba1 Rabbit pAb (A12391)

Western blot analysis of extracts of various cell lines, using Iba1 Rabbit pAb (A12391) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 120s.

ABclonal:Immunohistochemistry - Iba1 Rabbit pAb (A12391) }

Immunohistochemistry - Iba1 Rabbit pAb (A12391)

Immunohistochemistry of paraffin-embedded rat bone marrow using Iba1 antibody (A12391) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Iba1 Rabbit pAb (A12391) }

Immunohistochemistry - Iba1 Rabbit pAb (A12391)

Immunohistochemistry of paraffin-embedded mouse spleen using Iba1 antibody (A12391) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - Iba1 Rabbit pAb (A12391) }

Immunofluorescence - Iba1 Rabbit pAb (A12391)

Immunofluorescence analysis of rat brain using Iba1 Rabbit pAb (A12391) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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