ABclonal

HotStart Taq DNA Polymerase (Mg2+ Plus Buffer)

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Manufacturer  ABclonal

Cat. No. AB-RK20601 Datasheet

HotStart Taq DNA polymerase is an innovative antibody-modified thermostable enzyme. The activity of the enzyme is completely closed at room temperature, avoiding the non-specific amplification and primer dimer in the preparation of the sample and the first cycle reaction temperature rise, increasing the specificity of DNA amplification. When heated to 95 °C, the antibody bound to the enzyme was rapidly inactivated and did not affect the subsequent Taq DNA polymerase reaction. The enzyme has the advantages of good specificity, high sensitivity, good reproducibility and high amplification efficiency.

Components

HotStart Taq DNA Polymerase (5, 000 U/ml)

10X PCR Reaction Buffer, Mg2+ plus

 

Detailed information

Featured Specification
Product Source An E.coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1.
Applications Multiplex PCR, Specialty PCR, PCR , Routine PCR
Unit Definition One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 75°C.
Storage Conditions 10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Tween 20, 0.5% NP-40, 50% Glycerol, pH 7.4 @ 25°C
Storage Temperature -20°C
Reaction Conditions 1X PCR Reaction Buffer, Mg2+ plus
Reaction Buffer or Mix 1X PCR Reaction Buffer, Mg2+ plus:
20 mM Tris-HCl, 10 mM (NH4)2SO4, 10 mM KCl, 2 mM MgSO4, 0.1% Triton X-100, pH 8.8 @ 25°C
Heat Inactivation No
5' - 3' Exonuclease Yes
3' - 5' Exonuclease No
Strand Displacement +
Resulting Ends Single-base 3' Overhangs
Protein M.W. Theoretical 94000 daltons
Error Rate ~ 285x10-6 bases

 

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