HotStart Taq 2X PCR Master Mix with Dye

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Cat. NO. AB-RK20605 (datasheet)

HotStart Taq DNA polymerase is an innovative antibody-modified thermostable enzyme. The activity of the enzyme is completely closed at room temperature, avoiding the non-specific amplification and primer dimer in the preparation of the sample and the first cycle reaction temperature rise, increasing the specificity of DNA amplification.
HotStart Taq 2X Master Mix with Dye is an optimized ready-to-use solution containing HotStart Taq DNA Polymerase, dNTPs, MgCl2, KCI and stabilizers, as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates at ~10 bp. Both dyes are present in concentrations that do not mask comigrating DNA bands. It is ideally suited to routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA products. It can amplify up to 4 kb from complex genomic DNA or up to 5 kb from lambda DNA. Applicable to the PCR reaction, colony PCR, primer extension, etc.

Featured Specification
Applications Multiplex PCR, Specialty PCR, PCR , Routine PCR
Storage Temperature -20°C
Reaction Buffer or Mix 1X Master Mix Composition:
10 mM Tris-HCl, 1.5 mM MgCl2, 50 mM KCl, 0.08% IPGAL 630, 0.05% Tween 20, pH8.6@25°C; 200 uM dNTPs, 5% Glycerol, 25 U/ml HotStart Taq DNA Polymerase, 1X Xylene Cyanol, 1X Tartrazine.
Heat Inactivation No
5' - 3' Exonuclease Yes
3' - 5' Exonuclease No
Strand Displacement +
Resulting Ends Single-base 3' Overhangs
Error Rate ~ 285x10-6 bases